Rausch U P, Jordan M, Rödel F, Aigner T, Otterness I G, Beuscher N, Röllinghoff M, Beuscher H U
Institute for Clinical Microbiology, University of Erlangen/Nürnberg, Germany.
Cytokine. 1994 Sep;6(5):504-11. doi: 10.1016/1043-4666(94)90078-7.
Interleukin 1 (IL-1) gene expression was investigated in mice following oral infection with Yersinia enterocolitica 08. In Peyer's patches (PP), the primary site of bacterial invasion, induction of IL-1 alpha mRNA was delayed when compared to IL-1 beta mRNA. As shown by in situ hybridization. IL-1 alpha and IL-1 beta mRNA were found to be expressed within different cell types. These results indicate that expression of the two forms of IL-1 is regulated in a cell-specific manner at the transcriptional level. Moreover, IL-1 (alpha and beta) mRNA was increased in other organs such as spleen and lung. In spleens, IL-1 beta mRNA was found within the red pulp, and IL-1 alpha mRNA was located to the marginal zone confirming that differential expression of IL-1 alpha and IL-1 beta mRNA does not represent a tissue-specific event. However, as revealed by immunohistochemistry and measuring IL-1 activity in tissue homogenates, synthesis of IL-1 proteins was not detectable in spleens, unless mice were challenged with LPS. Because IL-1 synthesis was inducible in spleen cells following actinomycin D treatment, the results indicate that at distant sites of infection IL-1 (alpha and beta) mRNA is expressed but not translated into protein. It is concluded that cell-specific transcription of IL-1 alpha and IL-1 beta as well as dissociation between IL-1 mRNA and protein synthesis are two mechanisms effective in regulating the production of IL-1 during infection.
研究了小鼠经小肠结肠炎耶尔森氏菌08口服感染后白细胞介素1（IL-1）基因的表达情况。在细菌入侵的主要部位派伊尔氏结（PP）中，与IL-1β mRNA相比，IL-1α mRNA的诱导出现延迟。原位杂交显示，IL-1α和IL-1β mRNA在不同细胞类型中表达。这些结果表明，两种形式的IL-1在转录水平上以细胞特异性方式受到调控。此外，在脾脏和肺等其他器官中，IL-1（α和β）mRNA有所增加。在脾脏中，在红髓内发现了IL-1β mRNA，而IL-1α mRNA定位于边缘区，这证实了IL-1α和IL-1β mRNA的差异表达并非组织特异性事件。然而，免疫组织化学和组织匀浆中IL-1活性的测定结果显示，除非用脂多糖攻击小鼠，否则在脾脏中检测不到IL-1蛋白的合成。由于放线菌素D处理后脾脏细胞中IL-1的合成是可诱导的，结果表明在感染的远处部位，IL-1（α和β）mRNA表达但未翻译成蛋白质。得出的结论是，IL-1α和IL-1β的细胞特异性转录以及IL-1 mRNA与蛋白质合成之间的解离是感染期间有效调节IL-1产生的两种机制。
