Barbuto J A, Grimes W J, Hersh E M
Depto de Immunologia, ICB-USP, Brazil.
Cancer Immunol Immunother. 1995 Jan;40(1):31-6. doi: 10.1007/BF01517233.
Infiltrating B lymphocytes are found within tumors, where their role and the antigens they recognize are poorly defined. After in vitro expansion of these cells, we were able to detect the production of antibodies to tumor necrosis factor alpha (TNF) in 13 of 17 human tumors studied. These antibodies were detected by both enzyme-linked immunosorbent assay and by neutralization. Anti-TNF antibodies were not produced by resting peripheral blood B cells of normal subjects. However, anti-TNF antibodies were produced by B cells obtained from healthy individuals, after either in vivo or in vitro antigenic stimulation. This suggests that anti-TNF antibody production may constitute part of the overall B cell response to antigens. The intratumoral production of anti-TNF antibody may play a role in tumor/host interactions.
肿瘤内可发现浸润的B淋巴细胞，但其作用以及它们所识别的抗原尚不清楚。在体外扩增这些细胞后，我们在17例研究的人类肿瘤中的13例中检测到了针对肿瘤坏死因子α（TNF）的抗体产生。这些抗体通过酶联免疫吸附测定法和中和法均可检测到。正常受试者的静息外周血B细胞不产生抗TNF抗体。然而，健康个体的B细胞在体内或体外受到抗原刺激后会产生抗TNF抗体。这表明抗TNF抗体的产生可能构成B细胞对抗原整体反应的一部分。肿瘤内抗TNF抗体的产生可能在肿瘤/宿主相互作用中发挥作用。
