gyrB基因位于山羊支原体环状染色体上与复制起点相对的位置。
The gyrB gene lies opposite from the replication origin on the circular chromosome of Mycoplasma capricolum.
作者信息
Sano K, Miyata M
机构信息
Department of Biology, Faculty of Science, Osaka City University, Japan.
出版信息
Gene. 1994 Dec 30;151(1-2):181-3. doi: 10.1016/0378-1119(94)90653-x.
PMID:7828871
Abstract
Using oligodeoxyribonucleotide primers and the polymerase chain reaction, we cloned and sequenced a 3.2-kb region which contains the gyrB gene, encoding the beta-subunit of gyrase from Mycoplasma capricolum. We localized gyrB opposite from the chromosomal replication origin (ori) of M. capricolum by genomic Southern hybridization.
摘要
利用寡脱氧核糖核苷酸引物和聚合酶链反应，我们克隆并测序了一个3.2kb的区域，该区域包含编码山羊支原体gyraseβ亚基的gyrB基因。我们通过基因组Southern杂交将gyrB定位在山羊支原体染色体复制起点（ori）的对面。
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