A simple method for repeated sampling of lumbar cerebrospinal fluid in goats.

A technique has been developed for repeated sampling of cerebrospinal fluid from conscious goats by means of a catheter placed surgically in the subarachnoid space between the sixth and seventh lumbar vertebrae. Uncontaminated cerebrospinal fluid in excess of 1.0 ml could be obtained readily from the cannulated goats several times daily for up to 6 weeks. Cannulated goats did not appear stressed during, and in between, sampling periods.