核糖体蛋白S1对大肠杆菌K - 12温度敏感突变体中缺陷翻译的恢复作用：特性与遗传学研究
Restoration by ribosomal protein S1 of the defective translation in a temperature-sensitive mutant of Escherichia coli K-12: characterization and genetic studies.
作者信息
Ohsawa H, Maruo B
出版信息
J Bacteriol. 1976 Sep;127(3):1157-66. doi: 10.1128/jb.127.3.1157-1166.1976.
Abstract
A temperature-sensitive mutant of Escherichia coli was isolated that had a temperature-sensitive defect in ribosomal-wash protein(s) required for translation in vitro of E. coli endogenous messenger ribonucleic acid. It was found that 30S ribosomal protein S1 rescued the defect in the ribosomal-wash protein(s) of the mutant and that the complete restoration to the wild-type level was attained when 1 mol of protein S1 was added to 1 mol of 70S ribosome. The mutation, tss, causing such a defect was mapped at 21 min and was closely linked to the pyrD locus, the region of which was entirely different from that of the other genes coding for the many ribosomal proteins of E. coli. These results indicate that the gene specified by this mutation is involved in the function of the 30S ribosomal protein S1.
摘要
分离出了大肠杆菌的一种温度敏感突变体，该突变体在体外翻译大肠杆菌内源性信使核糖核酸所需的核糖体洗脱蛋白中存在温度敏感缺陷。发现30S核糖体蛋白S1挽救了突变体核糖体洗脱蛋白中的缺陷，并且当向1摩尔70S核糖体中加入1摩尔蛋白S1时，可完全恢复到野生型水平。导致这种缺陷的突变tss定位在21分钟处，并且与pyrD基因座紧密连锁，该区域与编码大肠杆菌许多核糖体蛋白的其他基因的区域完全不同。这些结果表明，由该突变指定的基因参与30S核糖体蛋白S1的功能。
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