大肠杆菌温度敏感型天冬酰胺基转移核糖核酸合成酶突变体的鉴定
Identification of a temperature-sensitive asparaginyl-transfer ribonucleic acid synthetase mutant of Escherichia coli.
作者信息
Yamamoto M, Nomura M, Ohsawa H, Maruo B
出版信息
J Bacteriol. 1977 Oct;132(1):127-31. doi: 10.1128/jb.132.1.127-131.1977.
Abstract
A temperature-sensitive mutant of Escherichia coli K-12 isolated previously (H. Ohsawa and B. Maruo, J. Bacteriol. 127:1157-1166, 1976) was found to have an alteration in asparaginyl-transfer ribonucleic acid synthetase. This alteration can account for the temperature-sensitive phenotype of the mutant. No evidence was obtained to support the previous suggestion that ribosomal protein S1 is altered in this mutant. Combined with the previous genetic studies, we conclude that the newly defined genetic locus, asnS, for the asparaginyl-transfer ribonucleic acid synthetase maps near pyrD at 21 min on the E. coli chromosome.
摘要
先前分离得到的大肠杆菌K-12的一个温度敏感突变体（大泽浩和丸尾邦，《细菌学杂志》127:1157 - 1166，1976年）被发现天冬酰胺基转移核糖核酸合成酶发生了改变。这种改变可以解释该突变体的温度敏感表型。没有获得证据支持之前关于该突变体中核糖体蛋白S1发生改变的推测。结合之前的遗传学研究，我们得出结论，新定义的负责天冬酰胺基转移核糖核酸合成酶的基因座asnS位于大肠杆菌染色体上21分钟处，靠近pyrD 。
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