• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

条件致死性核糖体蛋白突变体：50S亚基蛋白修饰所需位点的特征分析

Conditionally lethal ribosomal protein mutants: characterization of a locus required for modification of 50S subunit proteins.

作者信息

Kushner S R, Maples V F, Champney W S

出版信息

Proc Natl Acad Sci U S A. 1977 Feb;74(2):467-71. doi: 10.1073/pnas.74.2.467.

DOI:10.1073/pnas.74.2.467
PMID:322127
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC392310/
Abstract

Mutagenized P1 bacteriophage were used to transduce a marker (aroE) adjacent to the cluster or ribosomal protein genes located at 72 min on the Escherichia coli chromosome. Linked temperature-sensitive transductants were isolated and characterized. A mutant unable to grow at 44 degrees was found to be defective in protein synthesis both in vivo and in vitro. At the restrictive temperature mutant cells lost all polyribosomes. Analysis of the ribosomal proteins revealed alterations in at least four 50S subunit proteins. The mutation (called rimE1, ribosomal protein modification) mapped between rpsE and aroE. It is suggested that the rimE locus is the structural gene for an activity that modifies a selected number of ribosomal proteins.

摘要

用诱变的P1噬菌体转导位于大肠杆菌染色体72分钟处的核糖体蛋白基因簇附近的一个标记基因（aroE）。分离并鉴定了连锁的温度敏感转导子。发现一个在44℃不能生长的突变体在体内和体外的蛋白质合成均有缺陷。在限制温度下，突变体细胞失去了所有的多聚核糖体。对核糖体蛋白的分析显示至少四种50S亚基蛋白发生了改变。该突变（称为rimE1，核糖体蛋白修饰）定位于rpsE和aroE之间。提示rimE位点是一种修饰特定数量核糖体蛋白活性的结构基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/6fc5b1e06b4c/pnas00024-0084-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/02dcec35bc8a/pnas00024-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/cc506d076f7d/pnas00024-0084-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/546211d4c6f3/pnas00024-0084-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/6fc5b1e06b4c/pnas00024-0084-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/02dcec35bc8a/pnas00024-0084-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/cc506d076f7d/pnas00024-0084-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/546211d4c6f3/pnas00024-0084-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb25/392310/6fc5b1e06b4c/pnas00024-0084-d.jpg

相似文献

1
Conditionally lethal ribosomal protein mutants: characterization of a locus required for modification of 50S subunit proteins.条件致死性核糖体蛋白突变体：50S亚基蛋白修饰所需位点的特征分析
Proc Natl Acad Sci U S A. 1977 Feb;74(2):467-71. doi: 10.1073/pnas.74.2.467.
2
Localized mutagenesis of the aroE-strA section of the Escherichia coli chromosome coding for ribosomal proteins.对大肠杆菌染色体中编码核糖体蛋白的aroE-strA区段进行定位诱变。
Mol Gen Genet. 1975 Dec 1;141(3):207-11. doi: 10.1007/BF00341800.
3
New chromosomal location for structural genes of ribosomal proteins.核糖体蛋白结构基因的新染色体定位。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2765-9. doi: 10.1073/pnas.72.7.2765.
4
A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).一种影响核糖体蛋白S2（rpsB）编码基因的大肠杆菌条件致死突变。
J Mol Biol. 1979 Aug 5;132(2):219-33. doi: 10.1016/0022-2836(79)90392-9.
5
Organization of ribosomal protein genes in Escherichia coli. I. Physical structure of DNA from transducing lambda phages carrying genes from the aroE-str region.大肠杆菌核糖体蛋白基因的组织。I. 携带来自aroE-str区域基因的转导λ噬菌体DNA的物理结构。
J Mol Biol. 1976 Sep 25;106(3):817-35. doi: 10.1016/0022-2836(76)90267-9.
6
Organization of ribosomal protein genes in Escherichia coli. II. Mapping of ribosomal protein genes by in vitro synthesis of ribosomal proteins using DNA fragments of a transducing phage as templates.大肠杆菌核糖体蛋白基因的组织。II. 以转导噬菌体的DNA片段为模板，通过体外合成核糖体蛋白对核糖体蛋白基因进行定位。
J Mol Biol. 1976 Sep 25;106(3):837-55. doi: 10.1016/0022-2836(76)90268-0.
7
Cluster of genes in Escherichia coli for ribosomal proteins, ribosomal RNA, and RNA polymerase subunits.大肠杆菌中核糖体蛋白、核糖体RNA和RNA聚合酶亚基的基因簇。
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2743-7. doi: 10.1073/pnas.72.7.2743.
8
Cold-sensitive growth of a mutant of Escherichia coli with an altered ribosomal protein S8: analysis of revertants.核糖体蛋白 S8 发生改变的大肠杆菌突变体的冷敏感生长：回复突变体分析
Mol Gen Genet. 1977 Apr 29;152(3):331-6. doi: 10.1007/BF00693088.
9
Isolation of transducing phage carrying rps T, the structural gene for ribosomal protein S20.携带核糖体蛋白S20的结构基因rps T的转导噬菌体的分离。
Mol Gen Genet. 1976 Feb 27;144(1):115-8. doi: 10.1007/BF00277314.
10
Restoration by ribosomal protein S1 of the defective translation in a temperature-sensitive mutant of Escherichia coli K-12: characterization and genetic studies.核糖体蛋白S1对大肠杆菌K - 12温度敏感突变体中缺陷翻译的恢复作用：特性与遗传学研究
J Bacteriol. 1976 Sep;127(3):1157-66. doi: 10.1128/jb.127.3.1157-1166.1976.

引用本文的文献

1
Chemical modulators of ribosome biogenesis as biological probes.核糖体生物发生的化学调节剂作为生物探针。
Nat Chem Biol. 2015 Dec;11(12):924-32. doi: 10.1038/nchembio.1957. Epub 2015 Nov 17.
2
Inhibition of ribosomal subunit synthesis in Escherichia coli by the vanadyl ribonucleoside complex.钒酰核糖核苷复合物对大肠杆菌核糖体亚基合成的抑制作用。
Curr Microbiol. 2013 Aug;67(2):226-33. doi: 10.1007/s00284-013-0350-5. Epub 2013 Mar 20.
3
Impairment of ribosomal subunit synthesis in aminoglycoside-treated ribonuclease mutants of Escherichia coli.

本文引用的文献

1
CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI.氯霉素对大肠杆菌中β-半乳糖苷酶合成的促进性抑制作用
Proc Natl Acad Sci U S A. 1963 Mar;49(3):400-7. doi: 10.1073/pnas.49.3.400.
2
A proposal for a uniform nomenclature in bacterial genetics.细菌遗传学统一命名法的提议。
Genetics. 1966 Jul;54(1):61-76. doi: 10.1093/genetics/54.1.61.
3
Distribution and function of genes concerned with aromatic biosynthesis in Escherichia coli.大肠杆菌中与芳香族生物合成相关基因的分布与功能
在氨基糖苷类药物处理的大肠杆菌核糖核酸酶突变体中核糖体亚基合成的损伤。
Arch Microbiol. 2012 Dec;194(12):1033-41. doi: 10.1007/s00203-012-0839-5. Epub 2012 Aug 29.
4
The Escherichia coli mrsC gene is required for cell growth and mRNA decay.大肠杆菌的mrsC基因是细胞生长和mRNA降解所必需的。
J Bacteriol. 1998 Apr;180(7):1920-8. doi: 10.1128/JB.180.7.1920-1928.1998.
5
A temperature-sensitive mutant of Escherichia coli with an alteration in ribosomal protein L22.一种核糖体蛋白L22发生改变的大肠杆菌温度敏感突变体。
Genetica. 1994;94(1):17-25. doi: 10.1007/BF01429216.
6
Functions of the gene products of Escherichia coli.大肠杆菌基因产物的功能。
Microbiol Rev. 1993 Dec;57(4):862-952. doi: 10.1128/mr.57.4.862-952.1993.
7
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
8
Identification and genetic mapping of the structural gene for an essential Escherichia coli membrane protein.大肠杆菌一种必需膜蛋白结构基因的鉴定与遗传定位
J Bacteriol. 1989 Jan;171(1):565-8. doi: 10.1128/jb.171.1.565-568.1989.
9
A proposal for a uniform nomenclature for the genetics of bacterial protein synthesis.关于细菌蛋白质合成遗传学统一命名法的提议。
Mol Gen Genet. 1976 Aug 19;147(2):145-51. doi: 10.1007/BF00267566.
10
Localized mutagenesis for the isolation of temperature-sensitive mutants of Escherichia coli affected in protein synthesis.用于分离在蛋白质合成中受影响的大肠杆菌温度敏感突变体的定位诱变。
Genetics. 1979 Feb;91(2):215-27. doi: 10.1093/genetics/91.2.215.
J Bacteriol. 1966 Apr;91(4):1494-508. doi: 10.1128/jb.91.4.1494-1508.1966.
4
Polyribosomes of growing bacteria.生长中细菌的多核糖体
Science. 1967 Nov 3;158(3801):658-60. doi: 10.1126/science.158.3801.658.
5
Localized mutagenesis of any specific small region of the bacterial chromosome.细菌染色体任何特定小区域的定位诱变。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3158-62. doi: 10.1073/pnas.68.12.3158.
6
Ribosomal proteins. Protein compositions of biosynthetic precursors and artifical subparticles from ribosomal subunits in Escherichia coli K 12.核糖体蛋白。大肠杆菌K12核糖体亚基生物合成前体和人工亚颗粒的蛋白质组成。
Eur J Biochem. 1971 May 28;20(2):249-57. doi: 10.1111/j.1432-1033.1971.tb01388.x.
7
Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis.核糖体蛋白。十二。通过二维凝胶电泳测定大肠杆菌小核糖体亚基和大核糖体亚基中的蛋白数量。
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276-82. doi: 10.1073/pnas.67.3.1276.
8
Studies on the assembly of ribosomes in vivo.核糖体在体内组装的研究。
Cold Spring Harb Symp Quant Biol. 1969;34:69-75. doi: 10.1101/sqb.1969.034.01.011.
9
The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.大肠杆菌的核糖体蛋白。I. 30S核糖体蛋白的纯化。
Biochemistry. 1969 Jul;8(7):2897-905. doi: 10.1021/bi00835a031.
10
Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.大肠杆菌中某些导致重组缺陷的突变的基因定位。
J Bacteriol. 1969 Jan;97(1):244-9. doi: 10.1128/jb.97.1.244-249.1969.