一种具有温度敏感性链霉素蛋白的大肠杆菌突变体。
A mutant of Escherichia coli with temperature-sensitive streptomycin protein.
作者信息
Kang S S
出版信息
Proc Natl Acad Sci U S A. 1970 Mar;65(3):544-50. doi: 10.1073/pnas.65.3.544.
Abstract
The temperature-sensitive mutation in our Escherichia coli strain C1714 appears to be in a ribosomal protein. The mutation maps at the streptomycin locus. Protein synthesis in intact cells stops immediately when the temperature is shifted from 30 to 42 degrees C. Analysis of polyribosome distributions after shift-up suggests that initiation of protein synthesis is defective at the high temperature. In vitro protein synthesis in extracts from C1714 is temperature sensitive when directed by natural mRNA, but not when directed by poly U.
摘要
我们的大肠杆菌菌株C1714中的温度敏感突变似乎存在于一种核糖体蛋白中。该突变位于链霉素位点。当温度从30℃转移到42℃时，完整细胞中的蛋白质合成立即停止。温度升高后对多核糖体分布的分析表明，高温下蛋白质合成的起始存在缺陷。当由天然mRNA指导时，C1714提取物中的体外蛋白质合成对温度敏感，但由聚尿苷酸指导时则不敏感。
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本文引用的文献
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