利用羟胺体外诱变分离R质粒的温度敏感突变体。
Isolation of temperature-sensitive mutants of R plasmid by in vitro mutagenesis with hydroxylamine.
作者信息
Hashimoto T, Sekiguchi M
出版信息
J Bacteriol. 1976 Sep;127(3):1561-3. doi: 10.1128/jb.127.3.1561-1563.1976.
Abstract
Temperature-sensitive mutants were isolated from a tetracycline resistance plasmid, pSC101, after mutagenesis with hydroxylamine. They were divided into three classes according to their growth characteristics. They include mutants defective in the replication of plasmid deoxyribonucleic acid and strains having a mutation in the drug resistance gene.
摘要
在用羟胺诱变后，从四环素抗性质粒pSC101中分离出温度敏感突变体。根据它们的生长特性，将它们分为三类。它们包括质粒脱氧核糖核酸复制有缺陷的突变体和耐药基因发生突变的菌株。
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