Lu Z, Li Y, Zhang Y, Kutish G F, Rock D L, Van Etten J L
Plum Island Animal Disease Center, ARS, USDA, NAA, Greenport, New York 11944-0848.
Virology. 1995 Jan 10;206(1):339-52. doi: 10.1016/s0042-6822(95)80049-2.
Forty-five kilobases of DNA, including the previously sequenced 2.2-kb inverted repeat region, located at the left termini of the 330-kb Chlorella virus PBCV-1 genome were sequenced and analyzed. Eighty-five complete open reading frames (ORFs) larger than 195 nucleotides were identified. Thirty-seven of the 85 ORFs, which are densely packed on both strands of the DNA, were considered major ORFs. Fifteen of the major ORFs have similarity to genes in the databases, including bacterial glycerophosphoryl diester phosphodiesterase, bacteriophage T4 endonuclease V, D-isomer specific 2-hydroxyacid dehydrogenases, and beta-alanine synthetase and bacterial nitrilases. Two major ORFs resemble the virus major capsid protein. Three major ORFs contain three or more ankyrin-like repeat elements and four ORFs encode proline-rich proteins.
对位于330 kb小球藻病毒PBCV-1基因组左末端的45千碱基DNA进行了测序和分析，其中包括先前已测序的2.2千碱基反向重复区域。鉴定出85个大于195个核苷酸的完整开放阅读框（ORF）。85个ORF中的37个紧密排列在DNA的两条链上，被视为主要ORF。15个主要ORF与数据库中的基因相似，包括细菌甘油磷酸二酯磷酸二酯酶、噬菌体T4内切核酸酶V、D-异构体特异性2-羟基酸脱氢酶、β-丙氨酸合成酶和细菌腈水解酶。两个主要ORF类似于病毒主要衣壳蛋白。三个主要ORF包含三个或更多锚蛋白样重复元件，四个ORF编码富含脯氨酸的蛋白质。
