Lu Z, Li Y, Que Q, Kutish G F, Rock D L, Van Etten J L
Plum Island Animal Disease Center, ARS, USDA, NAA, Greenport, New York 11944-0848, USA.
Virology. 1996 Feb 1;216(1):102-23. doi: 10.1006/viro.1996.0038.
Analysis of 94 kb of DNA, located between map positions 88 and 182 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 195 open reading frames (ORFs) 65 codons or longer. One hundred and five of the 195 ORFs were considered major ORFs. Twenty-six of the 105 major ORFs resembled genes in the databases including three chitinases, a chitosanase, three serine/threonine protein kinases, two additional protein kinases, a tyrosine protein phosphatase, two ankyrins, an ornithine decarboxylase, a copper/zinc-superoxide dismutase, a proliferating cell nuclear antigen, a DNA polymerase, a fibronectin-binding protein, the yeast Ski2 protein, an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease, and an amidase. The genes for the 105 major ORFs were evenly distributed along the genome and, except for one noncoding 1788-nucleotide stretch, the genes were close together. Unexpectedly, a 900-bp region in the 1788-bp noncoding sequence resembled a CpG island.
对小球藻病毒PBCV-1基因组330kb中位于图谱位置88kb至182kb之间的94kb DNA进行分析，发现了195个65个密码子或更长的开放阅读框（ORF）。195个ORF中有105个被视为主要ORF。105个主要ORF中的26个与数据库中的基因相似，包括三种几丁质酶、一种壳聚糖酶、三种丝氨酸/苏氨酸蛋白激酶、另外两种蛋白激酶、一种酪氨酸蛋白磷酸酶、两种锚蛋白、一种鸟氨酸脱羧酶、一种铜/锌超氧化物歧化酶、一种增殖细胞核抗原、一种DNA聚合酶、一种纤连蛋白结合蛋白、酵母Ski2蛋白、一种腺嘌呤DNA甲基转移酶及其相应的DNA位点特异性内切酶，以及一种酰胺酶。105个主要ORF的基因沿基因组均匀分布，除了一段1788个核苷酸的非编码序列外，这些基因紧密相连。出乎意料的是，1788bp非编码序列中的一个900bp区域类似于一个CpG岛。
