Woodward T M, Carlson J O, de la Concha-Bermejillo A, DeMartini J C
Department of Pathology, Colorado State University, College of Veterinary Medicine and Biomedical Sciences, Fort Collins 80523.
J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Feb 1;8(2):124-33.
Ovine lentivirus (OvLV) strains vary in cytopathogenicity, but the correlation, if any, between viral cytophenotypes and in vivo pathogenicity is poorly understood. To examine this issue and to evaluate changes in OvLV strains following passage in vivo, biological and genetic characteristics of OvLV isolates following in vivo passage were compared with those of the parent strain used for inoculation of two sets of isogeneic twin lambs. Plaque-purified OvLV strains with "slow/low" (84/28) and "rapid/high" (85/34) cell culture phenotypes were used for inoculation of the lambs. The phenotypes of the parent OvLV strains were compared with virus isolates from the four lambs by assaying virus replication and cytopathogenicity in goat synovial membrane cells. Viral population genetic differences in the env region were compared by polymerase chain reaction (PCR) amplification and denaturing gradient gel electrophoresis (DGGE) of the fragments. Virus isolates recovered from rapid/high virus-infected sheep were more lytic and developed syncytia earlier than viruses reisolated from sheep inoculated with the slow/low strain. Isolates from lambs infected with 84/28 were more cytopathic in all assays than was their parent strain. Isolates from animals infected with 85/34 were more lytic and syncytiogenic than the parent strain, but responded similarly in replication assays. Although there were no consistent phenotypic differences between virus isolates recovered from sets of twins with markedly different degrees of lymphoid interstitial pneumonia (LIP), the DGGE band patterns of PCR amplified env fragments of the virus isolates from the twin lamb set with severe LIP, but not the set with slight LIP, were distinctly different from those of the parental viruses.
绵羊慢病毒（OvLV）毒株在细胞致病性方面存在差异，但对于病毒细胞表型与体内致病性之间（若存在）的相关性却知之甚少。为研究此问题并评估OvLV毒株在体内传代后的变化，将体内传代后的OvLV分离株的生物学和遗传学特征与用于接种两组同基因双羔羔羊的亲代毒株进行了比较。具有“慢/低”（84/28）和“快/高”（85/34）细胞培养表型的空斑纯化OvLV毒株用于接种羔羊。通过检测山羊滑膜细胞中的病毒复制和细胞致病性，将亲代OvLV毒株的表型与来自四只羔羊的病毒分离株进行比较。通过对片段进行聚合酶链反应（PCR）扩增和变性梯度凝胶电泳（DGGE），比较env区域的病毒群体遗传差异。从感染快/高病毒的绵羊中回收的病毒分离株比从接种慢/低毒株的绵羊中重新分离的病毒更具溶解性，且更早形成多核巨细胞。在所有检测中，来自感染84/28的羔羊的分离株比其亲代毒株更具细胞病变性。来自感染85/34的动物的分离株比亲代毒株更具溶解性和多核巨细胞形成能力，但在复制检测中的反应相似。尽管从患有明显不同程度淋巴间质性肺炎（LIP）的双胞胎组中回收的病毒分离株之间没有一致的表型差异，但来自患有严重LIP的双羔羔羊组而非轻度LIP组的病毒分离株的PCR扩增env片段的DGGE条带模式与亲代病毒明显不同。
