Zheng X, Chen Z, Xu W, Shi H
Institute of Acupuncture, China Academy of Traditional Chinese Medicine, Beijing.
Zhen Ci Yan Jiu. 1994;19(1):11-5.
The present experiments were designed to study the effect of glutamate on cortical somatosensory area II (S II) producing descending modulation of intralaminar nuclei (ILN) via the motor cortex (MCtx) in acupuncture analgesia. The glutamate antagonist glutamate diethylester (GDEE) or saline was topically applied at MCtx in 17 cats. Single unit activities of ILN neurons were extracellularly recorded. The results were as follows: 1. The nociceptive responses of ILN neurons were attenuated by stimulating S II after topical administration of saline at MCtx. However, the inhibitory effect of stimulating S II in the same neurons was reduced after application of GDEE. There was a significant difference at 0'-1' after the stimulation between the two groups (n = 10, P < 0.05). 2. The inhibitory effect of electroacupuncture (EA) on nociceptive responses was reduced after topical application of GDEE, while marked inhibition was shown at 0'-10' after cessation of EA in the saline control group (n = 11, P < 0.05). The results, together with the finding that the majority of S II neurons could be activated by EA, showed that glutamate released from S II to MCtx might be involved in corticofugal modulation of ILN from S II via MCtx in acupuncture analgesia.
本实验旨在研究谷氨酸对皮质体感区II（S II）的影响，该影响通过运动皮层（MCtx）在针刺镇痛中产生对板内核（ILN）的下行调制。在17只猫的MCtx局部应用谷氨酸拮抗剂谷氨酸二乙酯（GDEE）或生理盐水。细胞外记录ILN神经元的单位活动。结果如下：1. 在MCtx局部应用生理盐水后，刺激S II可使ILN神经元的伤害性反应减弱。然而，应用GDEE后，相同神经元中刺激S II的抑制作用减弱。两组在刺激后0'-1'时有显著差异（n = 10，P < 0.05）。2. 局部应用GDEE后，电针（EA）对伤害性反应的抑制作用减弱，而在生理盐水对照组中，电针停止后0'-10'时显示出明显的抑制作用（n = 11，P < 0.05）。这些结果，连同大多数S II神经元可被电针激活这一发现，表明在针刺镇痛中，从S II释放到MCtx的谷氨酸可能参与了S II通过MCtx对ILN的皮质传出调制。
