Yin G P, Su Y K, Zhuang G X
Jinan Minitary General Hospital.
Zhonghua Fu Chan Ke Za Zhi. 1994 Sep;29(9):538-41, 574.
The cellular DNA and RNA contents of 152 patients with hydatidiform moles (69 benign and 83 malignant) were analysed by flow cytometry (FCM), and strict follow-up was implemented. The chorionic villi of twelve cases in early pregnancy were used as normal control and another 12 cases of choriocarcinoma as positive control. Retrospective as well as prospective studies were conducted. The results showed that the accuracy rates of diagnosis of DNA aneuploid and RNA were respectively 66.7% and 82.6% in benign moles, 68.7% and 81.9% in invasive moles. Statistically the RNA parameter was significantly more sensitive the DNA parameter (P < 0.05). Analysis with both RNA and DNA parameters in predicting malignancy, the accuracy rate was 94.0%. Our study suggests that both the aneuploidy DNA and RNA contents may be important indicators for forecasting malignant change. The RNA parameter is more sensitive than the DNA parameter, and the use of both parameters is more accurate than either one alone. In addition, the concept of "standard DNA index" is raised.
采用流式细胞术（FCM）分析了152例葡萄胎患者（69例良性和83例恶性）的细胞DNA和RNA含量，并进行了严格的随访。将12例早孕绒毛用作正常对照，另12例绒毛膜癌用作阳性对照。进行了回顾性和前瞻性研究。结果显示，良性葡萄胎中DNA非整倍体和RNA诊断的准确率分别为66.7%和82.6%，侵蚀性葡萄胎中分别为68.7%和81.9%。统计学上，RNA参数比DNA参数更敏感（P<0.05）。用RNA和DNA参数联合预测恶性变，准确率为94.0%。我们的研究表明，DNA非整倍体和RNA含量可能都是预测恶变的重要指标。RNA参数比DNA参数更敏感，联合使用两个参数比单独使用任一参数更准确。此外，还提出了“标准DNA指数”的概念。
