大鼠哈德氏腺、视网膜和泪腺中存在抗利尿激素的免疫组织化学证据。

Immunohistochemical evidence for the presence of vasopressin in the rat harderian gland, retina and lacrimal gland.

作者信息

Djeridane Y

机构信息

Institute of Biology, University of Sciences and Technology, Houari Boumedienne, USTHB, Algiers, Algeria.

出版信息

Exp Eye Res. 1994 Jul;59(1):117-20. doi: 10.1006/exer.1994.1087.

DOI:10.1006/exer.1994.1087

PMID:7835392

Abstract

The presence of vasopressin in the Harderian gland, the retina and the lacrimal gland of the rat, has been examined immunohistochemically. After fixation in Bouin's solution, immunostaining is accomplished with the peroxidase-antiperoxidase method. In the retina, the immunoreactive vasopressin is almost exclusively restricted to the ganglion cell layer. In the Harderian gland, the immunoreactivity is localized in the epithelial cells of the excretory duct and restricted to the cytoplasm. In the lacrimal gland, the immunoreactivity is localized in the secretory cells of the acini and the intercalated ducts and the immunostaining is restricted to the cytoplasm surrounding the nucleus. The possible functions of vasopressin in these ocular structures are discussed.

摘要

已经采用免疫组织化学方法检测了大鼠哈德氏腺、视网膜和泪腺中抗利尿激素的存在情况。用布安氏溶液固定后，采用过氧化物酶-抗过氧化物酶方法进行免疫染色。在视网膜中，免疫反应性抗利尿激素几乎仅局限于神经节细胞层。在哈德氏腺中，免疫反应性定位于排泄管的上皮细胞，且局限于细胞质。在泪腺中，免疫反应性定位于腺泡和闰管的分泌细胞，免疫染色局限于细胞核周围的细胞质。文中讨论了抗利尿激素在这些眼部结构中的可能功能。