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Protein synthesis in collagen lattice-cultured fibroblasts is controlled at the ribosomal level.

作者信息

Gillery P, Georges N, Wegrowski J, Randoux A, Borel J P

机构信息

Laboratory of Biochemistry and Molecular Biology, Faculty of Medicine, University of Reims-Champagne-Ardenne, France.

出版信息

FEBS Lett. 1995 Jan 9;357(3):287-9. doi: 10.1016/0014-5793(94)01375-b.

DOI:10.1016/0014-5793(94)01375-b
PMID:7835429
Abstract

Fibroblasts cultivated in three-dimensional lattices exhibit a large decrease of protein synthesis, mainly through transcriptional control. However, no previous work was devoted to a potential ribosomal regulation. We evaluated ribosomal ribonucleic acid (RNA) in monolayer- and collagen lattice-cultured fibroblasts. After one week of culture, total RNA was 60% lower in lattice-cultured fibroblasts than in monolayer-cultured cells. The decrease was identical for 18 S and 28 S rRNA subfractions. The half-life of RNA was much shorter in collagen lattice-cultured fibroblasts than in monolayers. These results suggest that protein synthesis in lattice-cultured fibroblasts is partly regulated at the ribosomal level.

摘要

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