Saito T, Aran J M
Department of Otolaryngology, Fukui Medical School, Japan.
ORL J Otorhinolaryngol Relat Spec. 1994 Nov-Dec;56(6):310-4. doi: 10.1159/000276682.
The detection of platinum in cochlear outer hair cells (OHCs) and renal proximal tubule cells was attempted by X-ray microanalysis and ion microscopy. Platinum was not detected by X-ray microanalysis in the lysosomes of OHCs or proximal tubule cells after 10 days treatment with 1.5 mg/kg of cisplatin. In the guinea pigs treated with 1.5 mg/kg of cisplatin for 14 days, platinum was detected by a wave-length-dispersive microanalyzer in only the lysosomes of kidney fixed by freezing. Ion microscopy failed to detect platinum. Improvement of fixation methods, longer administration of cisplatin and the use of more sensitive tools will be needed to detect the platinum inside the OHCs.
通过X射线微分析和离子显微镜尝试检测耳蜗外毛细胞（OHCs）和肾近端小管细胞中的铂。在用1.5mg/kg顺铂处理10天后，X射线微分析未在OHCs或近端小管细胞的溶酶体中检测到铂。在用1.5mg/kg顺铂处理14天的豚鼠中，仅在冷冻固定的肾脏溶酶体中通过波长色散微分析仪检测到铂。离子显微镜未能检测到铂。需要改进固定方法、延长顺铂给药时间并使用更灵敏的工具来检测OHCs内的铂。
