Quintanilla-Martínez L, Gamboa-Domínguez A, Gámez-Ledesma I, Orozco-Estévez H, Angeles-Angeles A
Departamento de Patología, Instituto Nacional de la Nutrición Salvador Zubirán, México, D.F.
Rev Invest Clin. 1994 Sep-Oct;46(5):355-62.
The Epstein-Barr virus (EBV) has been demonstrated in association with cases of Hodgkin's disease (HD) indicating that, in at least in some cases, EBV may play a pathogenic role in the development of HD. To determine the prevalence of EBV in HD in a Mexican adult population, we studied 39 formalin-fixed and/or B-5, paraffin embedded samples of patients with HD, by immunoperoxidase; in situ hybridization was done in 32 of the 39 cases. We analyzed the presence of the latent membrane protein (LMP) and EBV-specific DNA sequences. Reed-Sternberg cells and mononuclear variants were positive for LMP in 28 cases (72%). LMP staining was found both on the cell surface and/or within the cytoplasm with enhancement in the Golgi area. The LMP was found in the single case of diffuse lymphocyte predominant HD, in 6/12 cases of nodular sclerosis (50%), in 10/15 cases of mixed cellularity (67%) and in all eleven cases of lymphocyte depleted HD (100%). No EBV-specific DNA sequences were found by in situ hybridization. Our results show: 1) a higher association of EBV in our population compared to the approximately 48% reported in developed countries; 2) all histologic subtypes of HD in Mexico appear to be strongly associated with EBV in contrast to the strong association with only mixed cellularity seen in Western populations; 3) the high prevalence of EBV in HD in Mexico may be a function of histology, ethnic groups, socioeconomic factors and/or geography.
已证实爱泼斯坦-巴尔病毒（EBV）与霍奇金淋巴瘤（HD）病例相关，这表明至少在某些情况下，EBV可能在HD的发生中起致病作用。为了确定墨西哥成年人群中HD患者EBV的感染率，我们采用免疫过氧化物酶法研究了39例HD患者经福尔马林固定和/或B-5固定、石蜡包埋的样本；39例中的32例进行了原位杂交。我们分析了潜伏膜蛋白（LMP）和EBV特异性DNA序列的存在情况。28例（72%）的里德-斯腾伯格细胞和单核细胞变异体LMP呈阳性。LMP染色在细胞表面和/或细胞质内均有发现，在高尔基体区域增强。在1例弥漫性淋巴细胞为主型HD中发现LMP，在12例结节硬化型HD中的6例（50%）、15例混合细胞型HD中的10例（67%）以及所有11例淋巴细胞消减型HD中均发现LMP（100%）。原位杂交未发现EBV特异性DNA序列。我们的结果显示：1）与发达国家报道的约48%相比，我们人群中EBV的关联度更高；2）与西方人群中仅混合细胞型HD与EBV有强关联不同，墨西哥HD的所有组织学亚型似乎都与EBV有强关联；3）墨西哥HD中EBV的高感染率可能是组织学、种族、社会经济因素和/或地理位置的作用。
