Tomita Y, Ohsawa M, Kanno H, Hashimoto M, Ohnishi A, Nakanishi H, Aozasa K
Department of Pathology, Osaka University Medical School, Japan.
Cancer. 1996 Jan 1;77(1):186-92. doi: 10.1002/(SICI)1097-0142(19960101)77:1<186::AID-CNCR30>3.0.CO;2-#.
The association of Epstein-Barr virus (EBV) and Hodgkin's disease (HD) has been suggested by serologic, epidemiologic, and molecular biologic studies. The high level of EBV association with HD in the developing countries was discussed in relation to the high HD incidence in these areas. Japanese HD shows a distinct peak incidence in older adults. In contrast, Western HD shows a bimodal pattern, the first peak in young adulthood and a second peak in older patients. In the present study, the EBV association with HD in Japan was investigated, and the results were compared with those reported from industrialized and developing countries.
Fifty-seven patients with HD were studied for the presence or absence of the EBV genome by the polymerase chain reaction and in situ hybridization methods. Seven cases were excluded from the analysis for EBV because of poor preservation of nucleotides in the specimens.
EBV genomes were detected in the Reed-Sternberg (R-S) cells of 32 of the 50 patients examined (64%). The EBV association was independently affected by histologic subtype (84% in mixed cellularity and 44% in others), sex (76% in males and 31% in females), and age (76% in patients aged 40 years and older and 38% in patients younger than 40 years of age; P < 0.01). High EBV association is found at the peak in older adults predominantly with mixed cellularity type. Previous studies revealed that the high EBV was associated with the older peak of the bimodal peaks in Western HD, and a unimodal peak in childhood in developing countries. The EBV subtype was predominantly type A, which is identical to the immunocompetent type of HD reported previously.
The results of the current study, together with those reported previously, showed that the presence of the Epstein-Barr virus correlated with mixed cellularity type, age older than 40 years, and male sex.
血清学、流行病学及分子生物学研究提示，EB病毒（EBV）与霍奇金淋巴瘤（HD）有关。鉴于发展中国家HD发病率较高，故对这些地区EBV与HD的高度相关性进行了讨论。日本HD的发病率在老年人中呈现明显的峰值。相比之下，西方HD呈现双峰模式，第一个峰值出现在青年期，第二个峰值出现在老年患者中。在本研究中，对日本HD与EBV的相关性进行了调查，并将结果与工业化国家和发展中国家报道的结果进行了比较。
采用聚合酶链反应和原位杂交方法，对57例HD患者进行EBV基因组检测。由于标本中核苷酸保存不佳，7例被排除在EBV分析之外。
在50例接受检查的患者中，有32例（64%）的里德-施特恩伯格（R-S）细胞中检测到EBV基因组。EBV相关性独立受组织学亚型（混合细胞型为84%，其他为44%）、性别（男性为76%，女性为31%）和年龄（40岁及以上患者为76% , 40岁以下患者为38%；P<0.01）影响。EBV高度相关性主要见于以混合细胞型为主的老年患者。既往研究显示，EBV高度相关性与西方HD双峰模式中的老年峰值以及发展中国家儿童期的单峰相关。EBV亚型主要为A型，与先前报道的免疫活性型HD相同。
本研究结果与先前报道的结果一致，表明EB病毒的存在与混合细胞型、40岁以上年龄及男性性别相关。
