Doering L C
Division of Anatomy, McMaster University, Hamilton, Ontario, Canada.
Bioessays. 1994 Nov;16(11):825-31. doi: 10.1002/bies.950161109.
New possibilities to modify function and direct repair in the central nervous system (CNS) have been established by the merger of gene transfer technology with neural transplantation. Rapid advances in viral-mediated DNA-delivery procedures permit the study of novel gene expression in neurons and glial cells. Foreign genes, transferred by a virus vector, can be used to generate new cell lines, identify transplanted cells, and express growth factors or enzymes for neurotransmitter synthesis. In addition to CNS cell types, non-neural cells are also being studied with transgene technology in the nervous system. Functional effects have been obtained with grafts of genetically modified cells in animal models of several nervous system disorders, and the recent results set the stage for potential application of these techniques to human CNS gene therapy.
基因转移技术与神经移植技术的融合，为中枢神经系统（CNS）功能的改变及直接修复带来了新的可能。病毒介导的DNA递送程序的快速发展，使得对神经元和神经胶质细胞中新型基因表达的研究成为可能。通过病毒载体转移的外源基因，可用于生成新的细胞系、识别移植细胞，以及表达用于神经递质合成的生长因子或酶。除了中枢神经系统细胞类型外，非神经细胞也正在通过转基因技术在神经系统中进行研究。在几种神经系统疾病的动物模型中，基因修饰细胞移植已取得了功能效应，最近的研究结果为这些技术在人类中枢神经系统基因治疗中的潜在应用奠定了基础。
