Phillips T M, Chmielinska J J
Department of Medicine, George Washington University Medical Center, Washington, DC.
Biomed Chromatogr. 1994 Sep-Oct;8(5):242-6. doi: 10.1002/bmc.1130080509.
An immunoaffinity capillary electrophoresis technique has been developed for the analysis of cyclosporin A in human tear fluid following topical application of the drug. The technique combines the selectivity of immunoaffinity separation with the high-resolution of capillary electrophoresis by immobilizing monoclonal antibody fragments directly onto the internal surface of the capillary. This technique was used to measure cyclosporin levels in tears obtained from corneal transplant patients during normal and drug toxicity episodes in the course of their treatment. Comparison of this technique with HPLC detection of cyclosporin in tears showed a good correlation, with the immunoaffinity CE technique having the advantage of being able to simultaneously detect toxic metabolites of cyclosporin in the same sample.
已开发出一种免疫亲和毛细管电泳技术，用于分析局部应用该药物后人泪液中的环孢素A。该技术通过将单克隆抗体片段直接固定在毛细管内表面，将免疫亲和分离的选择性与毛细管电泳的高分辨率相结合。该技术用于测量角膜移植患者在治疗过程中正常和药物毒性发作期间获得的泪液中环孢素水平。将该技术与泪液中环孢素的HPLC检测进行比较，结果显示具有良好的相关性，免疫亲和CE技术的优势在于能够在同一样品中同时检测环孢素的有毒代谢物。
