Zhang Y, Saito H, Nishiyama N
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Biol Pharm Bull. 1994 Sep;17(9):1199-205. doi: 10.1248/bpb.17.1199.
The subject mice were thymectomized 4 weeks after birth. Ten months after the thymectomy, learning behaviors in passive and active avoidance performances and a spatial memory task, the contents of brain monoamines and brain choline acetyltransferase (ChAT) activity, as well as the immune response were evaluated. DX-9386, a traditional Chinese medicinal prescription consisting of ginseng, polygala, acorus and hoelen, was prepared in CE-2 mouse food (1%, w/w) and given to the thymectomized mice after the operation until all the experiments were finished. DX-9386 treatment significantly ameliorated the learning and memory ability impaired by thymectomy in passive avoidance performances and in a spatial memory task, and the mice tended to improve in the active avoidance performance of a lever press test. However, DX-9386 treatment did not improve the thymectomy-reduced immune response. The contents of hypothalamic norepinephrine, 3,4-dihydroxyphenylacetic acid and homovanillic acid, and hypothalamic ChAT activity were significantly increased in thymectomized mice, and DX-9386 restored them to the control levels. These results suggested that DX-9386 mainly affected the cognitive process of the central nervous system to ameliorate the learning and memory deficit induced by thymectomy.
将出生4周后的实验小鼠进行胸腺切除。胸腺切除10个月后，评估其在被动和主动回避行为以及空间记忆任务中的学习行为、脑单胺含量、脑胆碱乙酰转移酶（ChAT）活性以及免疫反应。DX - 9386是一种由人参、远志、菖蒲和茯苓组成的中药方剂，以1%（w/w）的比例添加到CE - 2小鼠食物中，在手术后给予胸腺切除的小鼠，直至所有实验结束。DX - 9386治疗显著改善了胸腺切除所致的被动回避行为和空间记忆任务中受损的学习和记忆能力，并且小鼠在杠杆按压测试的主动回避行为上有改善趋势。然而，DX - 9386治疗并未改善胸腺切除所降低的免疫反应。胸腺切除小鼠下丘脑去甲肾上腺素、3,4 - 二羟基苯乙酸和高香草酸的含量以及下丘脑ChAT活性显著增加，而DX - 9386将它们恢复到对照水平。这些结果表明，DX - 9386主要影响中枢神经系统的认知过程，以改善胸腺切除所致的学习和记忆缺陷。
