Kaul S, Krishnakanth T P
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
Mol Cell Biochem. 1994 Aug 31;137(2):101-7. doi: 10.1007/BF00944071.
The effect of retinol deficiency and curcumin and turmeric feeding on brain microsomal Na(+)-K(+)ATPase activity was investigated. The brain Na(+)-K(+)ATPase activity registered an increase of 148.5% as compared to the control group. Upon treating retinol deficient rats with curcumin or turmeric, the abnormally elevated activity showed a decrease of 36.9 and 47.1%, respectively, when compared to the retinol deficient group. An increase in Vmax by 67% and Km by 66% for ATP was observed in the retinol deficient group. Curcumin or turmeric fed retinol-deficient groups reduced the Vmax by 25 and 33%, while Km was reduced by 25 and 31%, respectively, compared to the retinol deficient group. Arrhenius plot of Na(+)-K(+) ATPase showed a typical bi-phasic pattern in all the groups. Cholesterol:Phospholipid ratio showed a decrease in the retinol-deficient group by 67.8%, which showed a marked increase in curcumin or turmeric treated groups. Detergents could increase the Na(+)-K(+) ATPase activity more in the control group than in the retinol deficient groups. Curcumin or turmeric improved the detergent action on the enzyme. Subsequent freezing and thawing over a period of 30 min decreased the enzyme activity by 22.8% in the retinol deficient group compared to 15.9% decrease in the control group. Curcumin or turmeric treated groups showed a decrease in the enzyme activity by 22.0 and 19.2%, respectively, when compared to the zero time in each group. In the presence of concanavalin-A (Con-A) there was only 52.4% stimulation in the enzyme activity in retinol deficient groups, compared to 108.0% in the control group.(ABSTRACT TRUNCATED AT 250 WORDS)
研究了视黄醇缺乏以及喂食姜黄素和姜黄对脑微粒体钠钾ATP酶活性的影响。与对照组相比，脑钠钾ATP酶活性增加了148.5%。用姜黄素或姜黄处理视黄醇缺乏的大鼠后，与视黄醇缺乏组相比，异常升高的活性分别降低了36.9%和47.1%。视黄醇缺乏组中ATP的Vmax增加了67%，Km增加了66%。与视黄醇缺乏组相比，喂食姜黄素或姜黄的视黄醇缺乏组Vmax分别降低了25%和33%，而Km分别降低了25%和31%。钠钾ATP酶的阿累尼乌斯曲线在所有组中均呈现典型的双相模式。视黄醇缺乏组中胆固醇与磷脂的比例降低了67.8%，而在姜黄素或姜黄处理组中则显著增加。去污剂在对照组中比在视黄醇缺乏组中更能增加钠钾ATP酶活性。姜黄素或姜黄改善了去污剂对该酶的作用。在30分钟内进行后续的冻融处理后，视黄醇缺乏组的酶活性降低了22.8%，而对照组降低了15.9%。与每组的零时间相比，姜黄素或姜黄处理组的酶活性分别降低了22.0%和19.2%。在伴刀豆球蛋白A（Con-A）存在的情况下，视黄醇缺乏组中酶活性仅受到52.4%的刺激，而对照组为108.0%。（摘要截短于250字）
