Kaul S, Krishnakantha T P
Department of Nutrition and Food Safety, Central Food Technological Research Institute (CFTRI), Mysore, India.
Nahrung. 1993;37(1):35-40. doi: 10.1002/food.19930370107.
The investigations showed a significant decrease in the alkaline phosphatase activity of retinol deficient liver (48.6%), kidney (65.8%) and spleen (61.9%), as compared to the controls (100%). An increase in Vmax and Km by 12 to 51.5% and 90.4 to 189%, respectively, was observed in all the tissues in the retinol deficient group, as compared to the controls. Subsequent freezing and thawing reduced the activity of alkaline phosphatase by 22.5 to 35.8% in the experimental group; whereas the reduction in the control group ranged from 8.8 to 21.5%. In the presence of lectins and detergents the activity of alkaline phosphatase decreased in both the groups to different levels.
研究表明，与对照组（100%）相比，维生素A缺乏的肝脏（48.6%）、肾脏（65.8%）和脾脏（61.9%）中的碱性磷酸酶活性显著降低。与对照组相比，维生素A缺乏组的所有组织中Vmax和Km分别增加了12%至51.5%和90.4%至189%。随后的冻融使实验组碱性磷酸酶的活性降低了22.5%至35.8%；而对照组的降低幅度为8.8%至21.5%。在存在凝集素和去污剂的情况下，两组中碱性磷酸酶的活性均下降至不同水平。
