合成生物学方法可用于工程益生菌和人类微生物组的成员，以用于生物医学应用。
Synthetic Biology Approaches to Engineer Probiotics and Members of the Human Microbiota for Biomedical Applications.
机构信息
Department of Chemical and Biological Engineering, Tufts University, Medford, Massachusetts 02155, USA; email:
Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, North Carolina 27695, USA; email:
出版信息
Annu Rev Biomed Eng. 2018 Jun 4;20:277-300. doi: 10.1146/annurev-bioeng-062117-121019. Epub 2018 Mar 12.
Abstract
An increasing number of studies have strongly correlated the composition of the human microbiota with many human health conditions and, in several cases, have shown that manipulating the microbiota directly affects health. These insights have generated significant interest in engineering indigenous microbiota community members and nonresident probiotic bacteria as biotic diagnostics and therapeutics that can probe and improve human health. In this review, we discuss recent advances in synthetic biology to engineer commensal and probiotic lactic acid bacteria, bifidobacteria, and Bacteroides for these purposes, and we provide our perspective on the future potential of these technologies.
摘要
越来越多的研究强烈表明人类微生物组的组成与许多人类健康状况密切相关，并且在某些情况下表明直接操纵微生物组会影响健康。这些见解引起了人们对工程化本土微生物群成员和非驻留益生菌的浓厚兴趣，将其作为生物诊断和治疗方法，可用于探索和改善人类健康。在这篇综述中，我们讨论了合成生物学在为这些目的工程化共生和益生菌乳杆菌、双歧杆菌和拟杆菌方面的最新进展，并提供了我们对这些技术未来潜力的看法。
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本文引用的文献
1
In Vivo Biosensing: Progress and Perspectives.体内生物传感：进展与展望
ACS Sens. 2017 Mar 24;2(3):327-338. doi: 10.1021/acssensors.6b00834. Epub 2017 Feb 24.
2
Microbial biofilms and the human skin microbiome.微生物生物膜与人体皮肤微生物群
NPJ Biofilms Microbiomes. 2016 Nov 23;2:3. doi: 10.1038/s41522-016-0004-z. eCollection 2016.
3
Toward a genetic tool development pipeline for host-associated bacteria.面向宿主相关细菌的遗传工具开发管道。
Curr Opin Microbiol. 2017 Aug;38:156-164. doi: 10.1016/j.mib.2017.05.006. Epub 2017 Jun 15.
4
CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice.在肥胖小鼠实验模型中，补充CECT 7765可恢复改变的血管功能。
Int J Med Sci. 2017 Apr 8;14(5):444-451. doi: 10.7150/ijms.18354. eCollection 2017.
5
Genetically engineered probiotic for the treatment of phenylketonuria (PKU); assessment of a novel treatment in vitro and in the PAHenu2 mouse model of PKU.用于治疗苯丙酮尿症（PKU）的基因工程益生菌；在PKU的PAHenu2小鼠模型中进行的体外和体内新疗法评估
PLoS One. 2017 May 17;12(5):e0176286. doi: 10.1371/journal.pone.0176286. eCollection 2017.
6
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Nat Commun. 2017 Apr 11;8:15028. doi: 10.1038/ncomms15028.
7
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Biotechnol Adv. 2017 Jul;35(4):419-442. doi: 10.1016/j.biotechadv.2017.04.002. Epub 2017 Apr 7.
8
The Association of the Skin Microbiota With Health, Immunity, and Disease.皮肤微生物组与健康、免疫和疾病的关联。
Clin Pharmacol Ther. 2017 Jul;102(1):62-69. doi: 10.1002/cpt.698. Epub 2017 Jun 9.
9
Engineering bacterial thiosulfate and tetrathionate sensors for detecting gut inflammation.工程化用于检测肠道炎症的细菌硫代硫酸盐和连四硫酸盐传感器。
Mol Syst Biol. 2017 Apr 3;13(4):923. doi: 10.15252/msb.20167416.
10
Sensitizing pathogens to antibiotics using the CRISPR-Cas system.利用 CRISPR-Cas 系统使病原体对抗生素敏感。
Drug Resist Updat. 2017 Jan;30:1-6. doi: 10.1016/j.drup.2016.11.001. Epub 2016 Nov 27.
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