犬椎间盘同种异体移植的实验研究
Experimental study of intervertebral disc allografting in the dog.
作者信息
Katsuura A, Hukuda S
机构信息
Department of Orthopaedic Surgery, Shiga University of Medical Science, Shiga, Japan.
出版信息
Spine (Phila Pa 1976). 1994 Nov 1;19(21):2426-32. doi: 10.1097/00007632-199411000-00011.
STUDY DESIGN
An investigation of the use of intervertebral disc allografting as a potential alternative to intervertebral fusion was performed. In 13 adult mongrel dogs, intervertebral disc units cryopreserved after slow freezing were grafted.
OBJECTIVES
To evaluate the morphologic and biosynthetic damage caused by cryopreserving the canine intervertebral disc before allografting.
SUMMARY OF BACKGROUND DATA
Cryopreservation did not alter the normal histologic appearance. The metabolic activity, measured by 35S-sulfate incorporation, was observed to be 44% of the fresh control.
METHODS
Transplanted intervertebral discs were examined histologically and radiographically at 12, 24, and 48 weeks after surgery.
RESULTS
The allografted disc had preserved anular and nuclear architecture with moderate loss of chondrocytes 12 weeks after grafting. However, the intervertebral disc showed evidence of progressive disc degeneration 1 year after transplantation.
CONCLUSIONS
More investigation of storage methods is needed to enhance the viability of the intervertebral disc for successful intervertebral disc allografting.
研究设计
对同种异体椎间盘移植作为椎间融合潜在替代方法的应用进行了一项调查。在13只成年杂种犬中，移植了经缓慢冷冻后保存的椎间盘单元。
目的
评估同种异体移植前冷冻保存犬椎间盘所造成的形态学和生物合成损伤。
背景资料总结
冷冻保存未改变正常组织学外观。通过35S-硫酸盐掺入法测定的代谢活性，观察到为新鲜对照组的44%。
方法
在术后12周、24周和48周对移植的椎间盘进行组织学和放射学检查。
结果
同种异体移植的椎间盘在移植12周后保留了纤维环和髓核结构，但软骨细胞有中度损失。然而，移植1年后椎间盘显示出进行性椎间盘退变的迹象。
结论
需要对储存方法进行更多研究，以提高椎间盘的活力，实现成功的同种异体椎间盘移植。
Zotero 插件