Olson E J, Hanley E N, Rudert M J, Baratz M E
A. B. Ferguson Laboratory for Orthopaedic Research, Department of Orthopaedic Surgery, University of Pittsburgh, Pennsylvania.
Spine (Phila Pa 1976). 1991 Sep;16(9):1081-8. doi: 10.1097/00007632-199109000-00013.
Vertebral column allografts, with their intervertebral discs, were implanted into thoracic spine defects (T7-T9) in 11 dogs in an attempt to re-establish spinal stability and preserve spinal biomechanics. Before implantation, the allografts were harvested under sterile conditions from similar-sized dogs and deep frozen at -80 C. The animals were followed for 18 months postoperatively. Radiographs demonstrated gradual loss of intervertebral disc height. Biomechanical analysis showed that the dogs with allografts had no significant difference in spine stiffness compared with normal spines in compression, flexion, and extension testing. Control spines that had been fused were significantly stiffer than the allograft spines in all modes tested (P less than 0.05). Histologic analysis showed incorporation of the allograft but with incomplete revascularization of the allograft's eighth thoracic body. This investigation found that vertebral body allografts with intervertebral discs can function successfully for 18 months in a canine model. This research may assist in the development of physiologic treatment for spinal deficiencies in humans.
将带有椎间盘的脊柱同种异体骨植入11只犬的胸椎缺损处（T7 - T9），试图重建脊柱稳定性并保留脊柱生物力学。植入前，在无菌条件下从体型相似的犬身上获取同种异体骨，并在-80℃深度冷冻。术后对动物进行了18个月的随访。X线片显示椎间盘高度逐渐降低。生物力学分析表明，在压缩、屈曲和伸展测试中，接受同种异体骨移植的犬的脊柱刚度与正常脊柱相比无显著差异。在所有测试模式下，已融合的对照脊柱比同种异体骨移植的脊柱明显更硬（P小于0.05）。组织学分析显示同种异体骨已融合，但同种异体骨第八胸椎椎体的血管再生不完全。本研究发现，带有椎间盘的椎体同种异体骨在犬模型中可成功发挥功能18个月。该研究可能有助于开发针对人类脊柱缺陷的生理性治疗方法。
