Endresen M J, Tøsti E, Lorentzen B, Henriksen T
Department of Obstetrics and Gynecology, Aker University Hospital, Oslo, Norway.
Am J Obstet Gynecol. 1995 Jan;172(1 Pt 1):196-201. doi: 10.1016/0002-9378(95)90112-4.
The null hypothesis of this study was that sera of women with preeclampsia are not cytotoxic to endothelial cells in culture.
Endothelial cells were incubated in the presence of sera (30% vol/vol) of either preeclamptic patients (n = 11) or normal pregnant women (n = 11). Release of chromium 51 from prelabeled cells was measured after exposure to the different sera. Viability of the cells was evaluated by trypan blue exclusion and plating efficiencies. Deoxyribonucleic acid and protein synthesis were studied by measuring incorporation of tritiated thymidine and leucine into deoxyribonucleic acid and proteins, respectively. Cell growth was determined by monitoring the number of cells per culture dish during a 5-day incubation period.
Release of chromium 51 from endothelial cells incubated in the presence of sera from preeclamptic women was similar to controls (26.3% +/- 4.7% vs 26.7% +/- 2.5%). There was no difference in the number of trypan blue-positive cells in cultures incubated in the presence of sera from preeclamptic women and controls. Seeding the cells in either sera from preeclamptic or control women gave the same percentage of attached cells. Similarly, preincubation of endothelial cells with either one of the two sera resulted in the same number of attached cells when they were reseeded (45% +/- 6% vs 40% +/- 15%, respectively). Incubation of endothelial cells with sera from preeclamptic or control women affected neither deoxyribonucleic acid nor protein synthesis of the endothelial cells. Furthermore, cell proliferation was similar in cultures incubated with sera from preeclamptic women and controls.
No evidence was found that sera of women with preeclampsia are cytotoxic to endothelial cells in culture.
本研究的无效假设是子痫前期妇女的血清对培养中的内皮细胞无细胞毒性。
将内皮细胞与子痫前期患者（n = 11）或正常孕妇（n = 11）的血清（体积分数30%）一起孵育。在暴露于不同血清后，测量预先标记细胞中51铬的释放量。通过台盼蓝排斥法和接种效率评估细胞活力。分别通过测量氚标记胸腺嘧啶核苷和亮氨酸掺入脱氧核糖核酸和蛋白质中的量来研究脱氧核糖核酸和蛋白质合成。通过在5天孵育期内监测每个培养皿中的细胞数量来确定细胞生长情况。
在内皮细胞与子痫前期妇女血清一起孵育时，51铬的释放量与对照组相似（26.3%±4.7%对26.7%±2.5%）。在子痫前期妇女血清和对照组血清中孵育的培养物中，台盼蓝阳性细胞数量没有差异。将细胞接种于子痫前期妇女或对照妇女的血清中，贴壁细胞的百分比相同。同样，用两种血清之一对内皮细胞进行预孵育后，当再次接种时，贴壁细胞数量相同（分别为45%±6%对40%±15%）。用子痫前期妇女或对照妇女的血清孵育内皮细胞，对内皮细胞的脱氧核糖核酸和蛋白质合成均无影响。此外，用子痫前期妇女血清和对照组血清孵育的培养物中细胞增殖情况相似。
未发现子痫前期妇女的血清对培养中的内皮细胞具有细胞毒性的证据。
