Steven F S, Palcic B
Department of Biochemistry and Molecular Biology, School of Biological Sciences, University of Manchester, UK.
Anticancer Res. 1994 Sep-Oct;14(5A):2017-20.
The screening of cervical smears is concerned with the detection of abnormal epithelial cells which may be indicative of cervical intraepithelial neoplasia (CIN). Several types of cells in cervical smears possess a cell surface protease where isoenzymic forms of this enzyme can be differentially inhibited. Using this phenomenon a simple fluorescent technique has been developed in conjunction with differential competitive inhibition which enables abnormal cervical epithelial cells to bind the fluorescent probe whilst other cells do not bind the probe. The abnormal cells can then be located by fluorescence microscopy and their co-ordinates recorded for subsequent characterisation of these cells by nuclear analysis employing haematoxylin to stain the nuclei.
宫颈涂片筛查旨在检测可能提示宫颈上皮内瘤变（CIN）的异常上皮细胞。宫颈涂片中的几种细胞具有细胞表面蛋白酶，该酶的同工酶形式可被不同程度地抑制。利用这一现象，结合差异竞争性抑制开发了一种简单的荧光技术，该技术可使异常宫颈上皮细胞结合荧光探针，而其他细胞则不结合该探针。然后可以通过荧光显微镜定位异常细胞，并记录其坐标，以便随后通过苏木精染色细胞核的核分析来表征这些细胞。
