Sui S F, Wu H, Guo Y, Chen K S
Department of Biological Sciences & Biotechnology, Tsinghua University, Beijing, People's Republic of China.
J Biochem. 1994 Sep;116(3):482-7. doi: 10.1093/oxfordjournals.jbchem.a124550.
Two model systems were used to study the conformational changes of melittin upon insertion into phospholipid. The first model system is phospholipid monolayers with different surface pressure. The second one is small unilamellar vesicles at above or below their phase transition temperature. The conformational changes of melittin upon insertion were elucidated by employing a combination of monolayer technique, fluorescence technique, CD, and FTIR. The results showed that melittin adsorbed on the lipid layer surface contains less alpha-helix than its counterpart inserted into the lipid layer. As the penetration depth of melittin increases, more ordered structures (alpha-helix) appear. A possible molecular mechanism underlying melittin insertion is proposed.
使用了两种模型系统来研究蜂毒素插入磷脂时的构象变化。第一个模型系统是具有不同表面压力的磷脂单层。第二个是处于高于或低于其相变温度的小单层囊泡。通过结合单层技术、荧光技术、圆二色光谱（CD）和傅里叶变换红外光谱（FTIR）来阐明蜂毒素插入时的构象变化。结果表明，吸附在脂质层表面的蜂毒素所含的α-螺旋比插入脂质层中的蜂毒素少。随着蜂毒素穿透深度的增加，会出现更多有序结构（α-螺旋）。提出了蜂毒素插入的一种可能分子机制。
