Kobayashi T, Urabe K, Winder A, Tsukamoto K, Brewington T, Imokawa G, Potterf B, Hearing V J
Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Pigment Cell Res. 1994 Aug;7(4):227-34. doi: 10.1111/j.1600-0749.1994.tb00054.x.
Tyrosinase-related protein 1 (TRP1) maps to the brown locus in mice. Although the specific function of TRP1 has been in dispute, mutations in its structural gene result in the formation of brown rather than black melanin. We have investigated the melanogenic function of TRP1 by using immune-affinity purification of the protein and also by using transfection of its gene into fibroblasts to study its characteristics. We show that TRP1 has the ability to oxidize DHICA, a melanogenic intermediate derived from DOPAchrome. In addition, TRP1 has the ability to interact with tyrosinase and significantly stabilize the latter's catalytic function.
酪氨酸酶相关蛋白1（TRP1）定位于小鼠的棕色基因座。尽管TRP1的具体功能一直存在争议，但其结构基因突变会导致棕色而非黑色黑色素的形成。我们通过免疫亲和纯化该蛋白以及将其基因转染到成纤维细胞中以研究其特性，来探究TRP1的黑素生成功能。我们发现TRP1具有氧化二羟基吲哚羧酸（DHICA，一种源自多巴色素的黑素生成中间体）的能力。此外，TRP1能够与酪氨酸酶相互作用，并显著稳定后者的催化功能。
