Haimovici F, Anderson D J
Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Mar 1;8(3):236-8. doi: 10.1097/00042560-199503010-00003.
Sperm were detectable by microscopic examination in human cervicovaginal lavage (CVL) specimens < or = 8 h after intercourse, whereas an enzyme-linked immunosorbent assay using the monoclonal antibody MHS-5, specific for a seminal vesicle antigen present in semen detected semen at a concentration of 1:2,500,000 (0.00004%) in CVL specimens and was positive < or = 24 h following unprotected intercourse. We recommend the routine use of semen detection assays to reduce false-positive results attributable to semen contamination in assays of pathogens, antibodies, or other factors in cervicovaginal secretions.
性交后≤8小时,通过显微镜检查可在人宫颈阴道灌洗(CVL)标本中检测到精子,而使用对精液中存在的精囊抗原具有特异性的单克隆抗体MHS-5进行的酶联免疫吸附测定,在CVL标本中能检测到浓度为1:2,500,000(0.00004%)的精液,并且在无保护性交后≤24小时呈阳性。我们建议常规使用精液检测测定法,以减少宫颈阴道分泌物中病原体、抗体或其他因素检测中因精液污染导致的假阳性结果。