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RAPD reactions from crude plant DNA. Adding RNase A as a "helper enzyme".

作者信息

Heinze B

机构信息

Federal Forest Research Centre, Institute of Forest Genetics, Vienna, Austria.

出版信息

Mol Biotechnol. 1994 Jun;1(3):307-10. doi: 10.1007/BF02921697.

DOI:10.1007/BF02921697
PMID:7859166
Abstract

As opposed to standard polymerase chain reaction (PCR) using specific primers, genome analysis involving short random primers, for example RAPD, may yield inconsistent results if crude plant DNA preparations are used as the template. When RNase A, a thermostable enzyme, was added to such reactions, highly repeatable banding patterns were obtained from crude plant DNA, thus speeding up analyses substantially.

摘要

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本文引用的文献

1
Concentration of primer and template qualitatively affects products in random-amplified polymorphic DNA PCR.引物和模板的浓度会定性地影响随机扩增多态性DNA聚合酶链式反应中的产物。
Biotechniques. 1993 Mar;14(3):362-4.
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Artifactual variation in randomly amplified polymorphic DNA banding patterns.随机扩增多态性DNA条带模式中的人为变异。
Biotechniques. 1993 Feb;14(2):214-7.
3
Use of PCR primers containing a 3'-terminal ribose residue to prevent cross-contamination of amplified sequences.使用含有3'-末端核糖残基的PCR引物以防止扩增序列的交叉污染。
Nucleic Acids Res. 1993 Sep 11;21(18):4339-43. doi: 10.1093/nar/21.18.4339.
4
Pretreatment with RNase to improve PCR amplification of DNA using 10-mer primers.用核糖核酸酶进行预处理，以改善使用10聚体引物对DNA的聚合酶链式反应扩增。
Biotechniques. 1993 Jun;14(6):908-10.
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Reverse transcription of mRNA by Thermus aquaticus DNA polymerase followed by polymerase chain reaction amplification.嗜热水生菌DNA聚合酶对信使核糖核酸进行逆转录，随后进行聚合酶链式反应扩增。
Methods Enzymol. 1993;218:413-9. doi: 10.1016/0076-6879(93)18033-9.
6
Suppression of PCR amplification by high levels of RNA.高水平RNA对PCR扩增的抑制作用。
Biotechniques. 1993 Jan;14(1):24-5.
7
Fingerprinting genomes using PCR with arbitrary primers.使用任意引物通过聚合酶链反应对基因组进行指纹分析。
Nucleic Acids Res. 1990 Dec 25;18(24):7213-8. doi: 10.1093/nar/18.24.7213.
8
A simple and rapid method for the preparation of plant genomic DNA for PCR analysis.一种用于制备用于PCR分析的植物基因组DNA的简单快速方法。
Nucleic Acids Res. 1991 Mar 25;19(6):1349. doi: 10.1093/nar/19.6.1349.
9
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.用任意引物扩增的DNA多态性可作为遗传标记。
Nucleic Acids Res. 1990 Nov 25;18(22):6531-5. doi: 10.1093/nar/18.22.6531.
10
DNA amplification fingerprinting using very short arbitrary oligonucleotide primers.使用极短的任意寡核苷酸引物进行DNA扩增指纹分析。
Biotechnology (N Y). 1991 Jun;9(6):553-7. doi: 10.1038/nbt0691-553.