Kochevar I E, Harber L C
J Invest Dermatol. 1977 Mar;68(3):151-6. doi: 10.1111/1523-1747.ep12492475.
The photoreactions of 3,3',4',5-tetrachlorosalicylanilide anion (TCSA-) with two serum proteins were studied. TCSA- and anions of two similar compounds, N-ethyl-3,5-dichlorosalicylamide and salicylanilide, bind noncovalently to human serum albumin (HSA) without irradiation in aqueous pH 7.4 buffered solutions. TCSA- noncovalently bound to HSA yields three types of photoproducts when irradiated with ultraviolet light (lambda greater than 360 nm). A covalently bonded photoadduct between TCSA- and HSA is formed and histidines in HSA are chemically modified. In addition to these two types of photoproducts which involve HSA, two of the four TCSA- photoproducts which form when HSA is absent are also formed when the TCSA-/HSA complex is irradiated. The results presented indicate that not all proteins in the skin are capable of being the carrier protein in photoallergy of TCSA- and that cross reactivity to other halogenated salicylanilides can be explained by further photochemical reactions of TCSA- photoproducts.
研究了3,3',4',5-四氯水杨酰苯胺阴离子（TCSA-）与两种血清蛋白的光反应。在pH 7.4的水性缓冲溶液中，TCSA-以及两种类似化合物N-乙基-3,5-二氯水杨酰胺和水杨酰苯胺的阴离子在无光照的情况下非共价结合到人血清白蛋白（HSA）上。当用紫外线（波长大于360 nm）照射时，非共价结合到HSA上的TCSA-会产生三种类型的光产物。形成了TCSA-与HSA之间的共价键合光加合物，并且HSA中的组氨酸发生了化学修饰。除了这两种涉及HSA的光产物类型外，当照射TCSA-/HSA复合物时，在没有HSA的情况下形成的四种TCSA-光产物中的两种也会形成。给出的结果表明，皮肤中的并非所有蛋白质都能够成为TCSA-光过敏中的载体蛋白，并且对其他卤代水杨酰苯胺的交叉反应性可以通过TCSA-光产物的进一步光化学反应来解释。
