Drumheller P D, Hubbell J A
Department of Chemical Engineering, University of Texas at Austin 78712-1062.
Anal Biochem. 1994 Nov 1;222(2):380-8. doi: 10.1006/abio.1994.1506.
Polymer networks of poly(ethylene glycol) (PEG) in densely cross-linked matrices of acrylic acid (AA) and trimethylolpropane triacrylate were synthesized as biospecific cell adhesive substrates. Networks grafted with synthetic adhesion peptides produced substrates to investigate long-term, receptor-mediated cell/surface interactions, without nonspecific protein adsorption producing spurious adhesion signals. PEG rendered the networks very resistant to cell adhesion in vitro, and AA provided reactive carboxyl moieties for N-terminal grafting of peptides. Networks with higher mass fractions of AA had greater background cell adhesion, which diminished with higher mass fractions of PEG such that complete resistance to cell adhesion could be obtained. Networks grafted with inactive control peptides (GRGES or no peptide) remained completely cell nonadhesive in the presence of serum or even when preincubated with adhesion proteins, while networks grafted with bioadhesive peptides (GRGDS, GYIGSRY, or GREDVY) supported morphologically complete fibroblast adhesion. The amount of AA in the network readily controlled the amount of incorporated peptide. These networks may be suitable as analytical tools specifically to investigate long-term cell/substrate interactions in the presence of serum, yet without non-specific protein adsorption producing adhesion signals other than those immobilized for study.
在丙烯酸（AA）和三羟甲基丙烷三丙烯酸酯的密集交联基质中合成了聚乙二醇（PEG）聚合物网络，作为生物特异性细胞粘附底物。接枝有合成粘附肽的网络产生了用于研究长期受体介导的细胞/表面相互作用的底物，而不会因非特异性蛋白质吸附产生虚假粘附信号。PEG使网络在体外对细胞粘附具有很强的抗性，而AA为肽的N端接枝提供了反应性羧基部分。具有较高AA质量分数的网络具有更大的背景细胞粘附性，随着PEG质量分数的增加而降低，从而可以获得对细胞粘附的完全抗性。接枝有非活性对照肽（GRGES或无肽）的网络在存在血清的情况下甚至在与粘附蛋白预孵育时仍完全不粘附细胞，而接枝有生物粘附肽（GRGDS、GYIGSRY或GREDVY）的网络支持形态学上完全的成纤维细胞粘附。网络中AA的量很容易控制掺入肽的量。这些网络可能适合作为分析工具，专门用于研究在存在血清的情况下长期的细胞/底物相互作用，而不会因非特异性蛋白质吸附产生除固定用于研究的粘附信号之外的其他粘附信号。
