Kramer R A, Cameron J R, Davis R W
Cell. 1976 Jun;8(2):227-32. doi: 10.1016/0092-8674(76)90006-4.
We have developed an in situ hybridization technique which can be used to screen large numbers of hybrid bacteriophage for the presence of a particular inserted DNA sequence. Plaques of hybrid phage are formed on E. coli lawns on nitrocellulose filters, and their DNA is released, denatured, and fixed directly on the filters for hybridization to radioactive RNA probes. We have used this technique to isolate a number of hybrid bacteriophage lambda which contain EcoRl restriction fragments of the ribosomal RNA genes from yeast, and have examined the DNA from several of these phage.
我们已经开发出一种原位杂交技术，可用于筛选大量杂交噬菌体，以检测特定插入DNA序列的存在。杂交噬菌体的噬菌斑在硝酸纤维素滤膜上的大肠杆菌菌苔上形成，其DNA被释放、变性，并直接固定在滤膜上，用于与放射性RNA探针杂交。我们已使用该技术分离出一些含有来自酵母核糖体RNA基因的EcoRl限制片段的杂交λ噬菌体，并检测了其中几个噬菌体的DNA。
