采用反相高效液相色谱法测定人血浆中磷酸氟达拉滨的含量。

Determination of fludarabine phosphate in human plasma using reversed phase high-performance liquid chromatography.

作者信息

Misztal G, Paw B

机构信息

Department of Medicinal Chemistry, Medical Academy, Lublin, Poland.

出版信息

Pharmazie. 1996 Oct;51(10):733-4.

PMID:8941940

Abstract

A rapid, simple and accurate HPLC method for the determination of fludarabine phosphate in human plasma is presented. Fludarabine phosphate from plasma was successfully purified. The samples were chromatographed on a LiChrosorb RP-18 (10 microns) column after purification using a Bakerbond Spe column. The mobile phase was methanol/phosphoric buffer pH 4.15 (20:80). The detection was carried out at 262 nm. The method was tested for linearity (range from 100 to 900 ng/ml), recovery (ca. 99.8%) and precision (C.V. = 3.2%).

摘要

本文介绍了一种快速、简便且准确的用于测定人血浆中磷酸氟达拉滨的高效液相色谱法。血浆中的磷酸氟达拉滨被成功纯化。使用Bakerbond Spe柱纯化后，样品在LiChrosorb RP - 18（10微米）柱上进行色谱分析。流动相为甲醇/ pH 4.15的磷酸缓冲液（20:80）。检测波长为262 nm。该方法进行了线性测试（范围为100至900 ng/ml）、回收率测试（约99.8%）和精密度测试（变异系数= 3.2%）。