Fullerton M J, Funder J W
Baker Medical Research Institute, Prahan, Victoria, Australia.
Cardiovasc Res. 1994 Dec;28(12):1863-7. doi: 10.1093/cvr/28.12.1863.
Increased cardiac collagen is seen in uninephrectomised rats made hypertensive by aldosterone infusion and high salt, with the possibility of a direct effect of aldosterone at the level of the cardiac fibroblast. The aim of this study was to examine the effects of aldosterone, glucocorticoids, and angiotensin II on collagen production in a series of rat cardiac fibroblast cultures.
Cardiac fibroblast cultures were established from neonatal and adult rats of Sprague-Dawley, WKY, and SHR strains, with collagen synthesis measured by incorporation of [3H]proline into collagen. Cells were grown alone or co-cultured with cardiomyocytes, and exposed to aldosterone, glucocorticoids, or angiotensin II for 24-28 h before determination of [3H]proline incorporation.
Aldosterone did not affect production of collagen; in contrast, angiotensin II increased, and the glucocorticoid agonist RU28362 suppressed, collagen production in cultured cardiac fibroblasts in the various rat strains.
Aldosterone does not exert a direct effect on collagen synthesis in rat cardiac fibroblasts grown in culture. The increased cardiac collagen observed in vivo in aldosterone treated, salt loaded rats may thus represent secondary effects of aldosterone in this model.
在通过输注醛固酮和高盐饮食诱导高血压的单肾切除大鼠中，可见心脏胶原增加，醛固酮可能在心脏成纤维细胞水平产生直接作用。本研究的目的是在一系列大鼠心脏成纤维细胞培养物中，研究醛固酮、糖皮质激素和血管紧张素II对胶原产生的影响。
从Sprague-Dawley、WKY和SHR品系的新生大鼠和成年大鼠建立心脏成纤维细胞培养物，通过将[3H]脯氨酸掺入胶原中来测量胶原合成。细胞单独培养或与心肌细胞共培养，并在测定[3H]脯氨酸掺入之前，暴露于醛固酮、糖皮质激素或血管紧张素II 24 - 28小时。
醛固酮不影响胶原的产生；相反，血管紧张素II增加，糖皮质激素激动剂RU28362抑制了各种大鼠品系培养的心脏成纤维细胞中的胶原产生。
醛固酮对培养的大鼠心脏成纤维细胞中的胶原合成没有直接作用。因此，在醛固酮处理、高盐负荷的大鼠体内观察到的心脏胶原增加可能代表该模型中醛固酮的继发效应。
