Yang W, Shen Y, Zhang Z
Department of Parasitology, Medical College of Yangzhou University.
Zhonghua Yi Xue Za Zhi. 1994 Mar;74(3):155-7, 191.
The ELISA and Dot-ELISA with rabbit anti-Setaria labial-papillosa antibody were applied to determine circulating antigens of Filaria bancrofti. The total positive rate was 96% (24/25) in the sera of patients with microfilaremia from endemic areas, Anhui province. The total positive rate of urines was 60% (15/25) in the same patients. The positive rates of the milks were both 100% (4/4) in the same patients. The conformity rate was 100% and 75% respectively for positive sera and urines (15 cases), milks (4). Negative reactions were observed in the sera, urines and milks of all the 43 healthy people from non-endemic areas in Jiangsu province. Negative reactions were observed in the urines of all the 11 non-filaria cases harbouring intestinal nematodes. The positive rate detected with ELISA was 36% (4/11) in the patient sera that were negative when they were detected by Dot-ELISA. Negative reactions were detected with Dot-ELISA in the sera of all the 10 non-filaria patients infected with Paragonimus westermani. The results showed that circulating antigens of Filaria bancrofti exist not only in the serum but also in the urine and the milk.
应用兔抗唇乳突丝状虫抗体的酶联免疫吸附测定法（ELISA）和斑点酶联免疫吸附测定法（Dot - ELISA）检测班氏丝虫循环抗原。安徽省流行区微丝蚴血症患者血清的总阳性率为96%（24/25）。同一批患者尿液的总阳性率为60%（15/25）。同一批患者乳汁的阳性率均为100%（4/4）。阳性血清和尿液（15例）、乳汁（4例）的符合率分别为100%和75%。江苏省非流行区的43名健康人的血清、尿液和乳汁均呈阴性反应。11例肠道线虫感染者但非丝虫病患者的尿液均呈阴性反应。ELISA检测呈阴性的患者血清，用Dot - ELISA检测时阳性率为36%（4/11）。10例感染卫氏并殖吸虫的非丝虫病患者的血清用Dot - ELISA检测均呈阴性反应。结果表明，班氏丝虫循环抗原不仅存在于血清中，也存在于尿液和乳汁中。
