Zhang Y, Chen W, Li H, Xue H, Qiu L
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1995;13(1):57-60.
Nine rabbits infected with male S. japonicum cercariae (worm recovery 15-133, mean 53.1) and 9 rabbits infected with female cercariae (worm recovery 1-102, mean 36.1) were bled periodically until 1 year post-infection. The sera were tested by ELISA, LA and CHR for antibody detection. Results showed that the positive rates were 88.9%, 55.6% and 83.3%, respectively. The duration of positive reaction was 3-51 wk post-infection for ELISA, 4-17 wk for LA and 2-35 wk for CHR and the fluctuated results were obtained in ELISA while positive CHR and LA results usually remained stable. The above-mentioned results suggest that antibody detection methods should not be neglected due to its sensitivity although antigen detection methods are considered more favorable. For the surveillance of schistosomiasis in endemic areas where schistosomiasis is under control, antibody detection methods are still worthy of recommending for case finding of new infections.
9只感染日本血吸虫雄尾蚴的兔子（虫体回收率为15 - 133，平均为53.1）和9只感染雌尾蚴的兔子（虫体回收率为1 - 102，平均为36.1）在感染后定期采血，直至感染后1年。采用酶联免疫吸附测定（ELISA）、乳胶凝集试验（LA）和环卵沉淀试验（CHR）检测血清中的抗体。结果显示，阳性率分别为88.9%、55.6%和83.3%。ELISA法感染后阳性反应持续时间为3 - 51周，LA法为4 - 17周，CHR法为2 - 35周，ELISA结果出现波动，而CHR和LA阳性结果通常保持稳定。上述结果表明，尽管抗原检测方法被认为更有利，但抗体检测方法因其敏感性也不应被忽视。对于血吸虫病已得到控制的流行区的血吸虫病监测，抗体检测方法仍值得推荐用于发现新感染病例。
