Antonakopoulos G N, Darnton S J, Newman J, Duffy J P, Matthews H R
Department of Histopathology, Birmingham Heartlands Hospital, UK.
Histopathology. 1994 Nov;25(5):447-54. doi: 10.1111/j.1365-2559.1994.tb00006.x.
Seven oesophageal squamous carcinomas, treated with pre-operative chemotherapy (mitomycin-C, ifosfamide and cisplatin-MIC), with a course finishing 21 days prior to resection, were examined by electronmicroscopy. In one treated case detailed light microscopy failed to reveal any tumour. Five of the remaining six tumours showed cytotoxic damage in that apoptosis and unusual necrotic changes were observed in almost all the neoplastic cells. These features were not seen in untreated cases. In four additional cases, who received one pulse of MIC followed by biopsy or resection within 3-6 days, apoptotic changes were very pronounced and extensive and most neoplastic cells presented unusual degeneration with characteristic derangement of the cytoskeleton, destruction of organelles and accumulation of glycogen. The ultrastructural appearance of 18 untreated resected oesophageal squamous carcinomas was studied for comparison with the treated tumours. The study has demonstrated ultrastructural changes resulting from chemotherapy. Results suggest that the regimen is more effective against squamous carcinomas than against adenocarcinomas of the oesophagus, as judged by comparison with the results of a previous study.
对7例接受术前化疗（丝裂霉素-C、异环磷酰胺和顺铂-MIC）的食管鳞状细胞癌进行了电子显微镜检查，化疗疗程在切除前21天结束。在1例接受治疗的病例中，详细的光学显微镜检查未发现任何肿瘤。其余6例肿瘤中有5例显示出细胞毒性损伤，几乎所有肿瘤细胞均观察到凋亡和异常坏死变化。这些特征在未治疗的病例中未见。在另外4例病例中，他们在接受1个疗程的MIC治疗后3至6天内进行活检或切除，凋亡变化非常明显且广泛，大多数肿瘤细胞呈现异常变性，伴有细胞骨架特征性紊乱、细胞器破坏和糖原积累。为了与接受治疗的肿瘤进行比较，研究了18例未治疗的切除食管鳞状细胞癌的超微结构外观。该研究证明了化疗引起的超微结构变化。结果表明，与先前研究结果相比，该方案对食管鳞状细胞癌比对腺癌更有效。
