Oguma K, Iida H, Shiozaki M
Infect Immun. 1976 Sep;14(3):597-602. doi: 10.1128/iai.14.3.597-602.1976.
Five toxigenic strains of Clostridium botulinum types C and D were incubated at 37 degrees C for 7 days in 15 ml of the following media: LYG medium, cooked-meat medium, egg meat medium, and N-Z-amine medium. The supernatants of these cultures were tested for hemagglutinin production with 1% erythrocytes obtained from mice, guinea pigs, chickens, sheep, monkeys, and humans. Four toxigenic strains produced hemagglutinin. The highest hemagglutinin titer was obtained with a combination of human erythrocytes and cultures incubated in LYG medium. When the same experiment was carried out with many nontoxigenic strains, hemagglutination was observed in only one strain, C-N71. Strains producing hemagglutinin also produced phages. The phages obtained from toxin- and hemagglutinin-producing strains converted nontoxigenic indicator strains to produce both toxin and hemagglutinin. The phage obtained from a toxin-positive hemagglutinin-negative strain could only induce cultures to produce toxin, and the phage from a toxin-negative hemagglutinin-positive strain could only induce production of hemagglutinin. These studies suggest that the production of hemagglutinin by C. botulinum types C and D is governed by bacteriophages and that hemagglutinin production can be transmitted separately or concomitantly with toxin production.
将5株C型和D型肉毒梭菌产毒株在37℃下于15毫升以下培养基中培养7天：LYG培养基、熟肉培养基、蛋肉培养基和N-Z-胺培养基。用从小鼠、豚鼠、鸡、绵羊、猴子和人类获得的1%红细胞检测这些培养物的上清液中血凝素的产生情况。4株产毒株产生了血凝素。用人红细胞与在LYG培养基中培养的培养物组合获得了最高的血凝素滴度。当用许多非产毒株进行相同实验时，仅在一株C-N71菌株中观察到了血凝现象。产生血凝素的菌株也产生噬菌体。从产生毒素和血凝素的菌株中获得的噬菌体可使非产毒指示菌株产生毒素和血凝素。从毒素阳性血凝素阴性菌株获得的噬菌体只能诱导培养物产生毒素，而从毒素阴性血凝素阳性菌株获得的噬菌体只能诱导血凝素的产生。这些研究表明，C型和D型肉毒梭菌血凝素的产生受噬菌体控制，并且血凝素的产生可以与毒素产生分开或同时传播。
