Elsässer-Beile U, von Kleist S, Gerlach S, Gallati H, Mönting J S
Institute of Immunobiology, Medical Faculty, University of Freiburg, Germany.
J Clin Lab Anal. 1994;8(6):447-51. doi: 10.1002/jcla.1860080618.
Levels of the cytokines interleukin-1-alpha, -1-beta, and -2 (IL-1-alpha, IL-1-beta, IL-2), tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) were measured in the mitogen-stimulated whole blood cell cultures from 96 patients with Crohn's disease (48 untreated, 12 treated with sulfasalazine, 36 treated with corticosteroids), 74 patients with ulcerative colitis (21 untreated, 25 treated with sulfasalazine, 28 steroid treated), and 360 healthy controls. The cytokines were measured 4 days after induction by a sensitive immunoenzyme assay. In the blood cell cultures of the untreated and sulfasalazine treated patients with Crohn's disease and ulcerative colitis higher levels of TNF-alpha, IL-1-alpha and IL-1-beta were found whereas IL-2 production was decreased and IFN-gamma-production was not significantly different as compared to the controls. Leukocytes of the corticosteroid-treated patients with both diagnoses showed a lower production of all measured cytokines compared to the untreated patients. The same results were obtained, when the somewhat different counts of mononuclear cells in the peripheral blood of the patients and controls were taken into account. The elevated production of proinflammatory cytokines in the blood cell cultures suggests a systemic immune activation in patients with inflammatory bowel disease.
对96例克罗恩病患者（48例未治疗、12例用柳氮磺胺吡啶治疗、36例用皮质类固醇治疗）、74例溃疡性结肠炎患者（21例未治疗、25例用柳氮磺胺吡啶治疗、28例用类固醇治疗）以及360例健康对照者经丝裂原刺激的全血细胞培养物中细胞因子白细胞介素-1-α、-1-β和-2（IL-1-α、IL-1-β、IL-2）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的水平进行了检测。在诱导4天后，采用灵敏的免疫酶测定法对细胞因子进行检测。在未治疗及用柳氮磺胺吡啶治疗的克罗恩病和溃疡性结肠炎患者的血细胞培养物中，发现TNF-α、IL-1-α和IL-1-β水平较高，而IL-2生成减少，且与对照相比，IFN-γ生成无显著差异。两种诊断的用皮质类固醇治疗的患者的白细胞与未治疗患者相比，所有检测的细胞因子生成均较低。当考虑患者和对照外周血中单核细胞计数略有差异时，也得到了相同的结果。血细胞培养物中促炎细胞因子生成增加提示炎症性肠病患者存在全身免疫激活。
