从人心脏cDNA文库中鉴定编码6-磷酸葡萄糖酸脱氢酶的cDNA。
Identification of a cDNA encoding 6-phosphogluconate dehydrogenase from a human heart cDNA library.
作者信息
Tsui S K, Chan J Y, Waye M M, Fung K P, Lee C Y
机构信息
Department of Biochemistry, Chinese University of Hong Kong, Shatin, N.T., Hong Kong.
出版信息
Biochem Genet. 1996 Oct;34(9-10):367-73. doi: 10.1007/BF00554412.
A full-length cDNA clone for human 6-phosphogluconate dehydrogenase (PGD) was isolated from a human adult heart cDNA library. The clone encoded an open reading frame of 483 amino acids. When the amino acid sequences of human PGD and sheep PGD were aligned, 94.2% identity between these two proteins was found. Its calculated molecular weight is 53,149 daltons. The predicted isoelectric point is 6.85. When the secondary structure of human PGD was examined by the PROSIS software, 36% alpha-helix and 9% beta-sheet were found.
从人成人心脏cDNA文库中分离出人类6-磷酸葡萄糖酸脱氢酶（PGD）的全长cDNA克隆。该克隆编码一个483个氨基酸的开放阅读框。当将人PGD和绵羊PGD的氨基酸序列进行比对时，发现这两种蛋白质之间的同一性为94.2%。其计算分子量为53,149道尔顿。预测的等电点为6.85。当使用PROSIS软件检查人PGD的二级结构时，发现其α-螺旋占36%，β-折叠占9%。
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