低严谨度聚合酶链反应（LS-PCR）可实现完全内部标准化的DNA定量。 - Suppr

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超能文献

Low stringency-PCR (LS-PCR) allows entirely internally standardized DNA quantitation.

作者信息

Caballero O L, Villa L L, Simpson A J

机构信息

Ludwig Institute for Cancer Research, São Paulo, Brazil.

出版信息

Nucleic Acids Res. 1995 Jan 11;23(1):192-3. doi: 10.1093/nar/23.1.192.

DOI:10.1093/nar/23.1.192

PMID:7870586

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306649/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bebd/306649/1d68613dd034/nar00001-0214-a.jpg

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本文引用的文献

Absolute quantification of target DNA: a simple competitive PCR for efficient analysis of multiple samples.靶DNA的绝对定量：一种用于高效分析多个样本的简单竞争性PCR。

Nucleic Acids Res. 1993 Apr 25;21(8):2017-8. doi: 10.1093/nar/21.8.2017.

Sex determination by low stringency PCR (LS-PCR).通过低严格度聚合酶链反应（LS-PCR）进行性别鉴定。

Nucleic Acids Res. 1993 Feb 11;21(3):763-4. doi: 10.1093/nar/21.3.763.

Definition of human papillomavirus type 16 DNA levels in low and high grade cervical lesions by a simple polymerase chain reaction technique.

Arch Virol. 1993;128(1-2):123-33. doi: 10.1007/BF01309793.

Genome amplification of human papillomavirus types 16 and 18 in cervical carcinomas is related to the retention of E1/E2 genes.

Int J Cancer. 1994 Mar 1;56(5):640-5. doi: 10.1002/ijc.2910560506.

Low-stringency PCR with diagnostically useful primers for identification of Leptospira serovars.使用具有诊断价值的引物进行低严谨度聚合酶链反应以鉴定钩端螺旋体血清型。

J Clin Microbiol. 1994 May;32(5):1369-72. doi: 10.1128/jcm.32.5.1369-1372.1994.

Low-stringency PCR provides an internal control for negative results in PCR-based diagnosis.低严谨度PCR为基于PCR的诊断中的阴性结果提供了内部对照。

PCR Methods Appl. 1994 Apr;3(5):305-7. doi: 10.1101/gr.3.5.305.

Rapid silver staining and recovery of PCR products separated on polyacrylamide gels.快速银染及聚丙烯酰胺凝胶上分离的PCR产物回收

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