Yee C, Krishnan-Hewlett I, Baker C C, Schlegel R, Howley P M
Am J Pathol. 1985 Jun;119(3):361-6.
A series of human carcinoma cell lines was examined for human papillomavirus (HPV) DNA sequences with the use of HPV-6, HPV-11, HPV-16, and HPV-18 DNA probes. Six of eight cell lines derived from human cervical carcinomas were shown to contain integrated HPV DNA sequences. In five of these six lines, HPV-specific polyadenylated RNA species could also be identified. The expression of HPV sequences was detected in three lines with a HPV-18 DNA probe and in two lines with a HPV-16 DNA probe. Of the two lines which contained HPV-16 specific RNA, one contained HPV DNA sequences which hybridized only to an HPV-16 probe, and the other contained HPV DNA sequences which hybridized to both HPV-16 and HPV-18 DNA probes. Six cell lines established from human squamous-cell carcinomas of the bladder, pharynx, lung, esophagus, and vulva were negative for HPV-6, HPV-11, HPV-16, and HPV-18 DNA sequences under stringent hybridization conditions.
使用人乳头瘤病毒（HPV）-6、HPV-11、HPV-16和HPV-18 DNA探针，对一系列人类癌细胞系进行了人乳头瘤病毒（HPV）DNA序列检测。来自人宫颈癌的8个细胞系中，有6个显示含有整合的HPV DNA序列。在这6个细胞系中的5个中，还能鉴定出HPV特异性的聚腺苷酸化RNA种类。用HPV-18 DNA探针在3个细胞系中检测到了HPV序列的表达，用HPV-16 DNA探针在2个细胞系中检测到了HPV序列的表达。在含有HPV-16特异性RNA的2个细胞系中，一个含有仅与HPV-16探针杂交的HPV DNA序列，另一个含有与HPV-16和HPV-18 DNA探针均杂交的HPV DNA序列。在严格杂交条件下，从人膀胱、咽、肺、食管和外阴鳞状细胞癌建立的6个细胞系，HPV-6、HPV-11、HPV-16和HPV-18 DNA序列均为阴性。
