Hatada I, Kitagawa K, Yamaoka T, Wang X, Arai Y, Hashido K, Ohishi S, Masuda J, Ogata J, Mukai T
National Cardiovascular Center Research Institute, Osaka, Japan.
Nucleic Acids Res. 1995 Jan 11;23(1):36-41. doi: 10.1093/nar/23.1.36.
The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, but established between 1.5 d.p.c. and 12.5 d.p.c. on the contrary, the allele-specific expression occurred in the two-cell stage when transcription initiates. Absence of the methylation of the upstream region in this stage indicates that methylation is not necessary for inactivation of the expression.
通过二维基因组扫描方法克隆的小鼠U2af1-rs1(SP2)基因仅从父本遗传的染色体表达。该基因与U2AF具有显著相似性，位于11号染色体上，母本重复/父本缺失会导致体型变小。在本报告中，我们克隆了基因组U2af1-rs1(SP2)，发现其启动子以母本等位基因特异性方式甲基化。这种等位基因甲基化在亲代配子中未建立，但在妊娠第1.5天至12.5天之间建立。相反，等位基因特异性表达在转录开始的二细胞阶段发生。此阶段上游区域甲基化的缺失表明甲基化对于表达的失活不是必需的。
