Miller V, Muirden K D
University of Melbourne, Department of Medicine, Royal Melbourne Hospital, Victoria, Australia.
Rheumatol Int. 1994;14(4):149-54. doi: 10.1007/BF00579700.
A radioreceptor assay for tumour necrosis factor alpha (TNF alpha)-binding proteins was development that is suitable for use with synovial fluids and sera. This assay, an alternative to the commonly used enzyme-linked immunosorbent assays (ELISAs), is not specific for soluble tumour necrosis factor alpha receptors (sTNF-R), but detects any molecules that might compete with TNF alpha for receptor binding. It also detects molecules that might bind TNF alpha and thereby interfere with subsequent binding to receptor. In a preliminary study, the assay was used to determine levels of TNF alpha-binding activity in a test group of synovial fluids from patients with rheumatoid arthritis (RA), osteoarthritis (OA) or psoriatic arthritis (PA). Levels of binding activity were much higher than those reported for sTNF-R alone in other studies [1, 2]. Our results indicated that there may be other molecules associated with the inflamed synovium that can interfere with the binding of TNF to its receptors and so attenuate its effect in diseases such as RA.
已开发出一种用于肿瘤坏死因子α（TNFα）结合蛋白的放射受体测定法，该方法适用于滑膜液和血清。这种测定法是常用酶联免疫吸附测定法（ELISA）的替代方法，它并非对可溶性肿瘤坏死因子α受体（sTNF-R）具有特异性，而是能检测任何可能与TNFα竞争受体结合的分子。它还能检测可能结合TNFα从而干扰其后续与受体结合的分子。在一项初步研究中，该测定法用于确定类风湿性关节炎（RA）、骨关节炎（OA）或银屑病关节炎（PA）患者滑膜液测试组中TNFα结合活性的水平。结合活性水平远高于其他研究中单独报道的sTNF-R水平[1, 2]。我们的结果表明，可能存在与炎症滑膜相关的其他分子，它们可干扰TNF与其受体的结合，从而在诸如RA等疾病中减弱其作用。
