健康人和内毒素血症患者血液样本中肿瘤坏死因子可溶性受体p55的检测

Detection of tumor necrosis factor soluble receptor p55 in blood samples from healthy and endotoxemic humans.

作者信息

Shapiro L, Clark B D, Orencole S F, Poutsiaka D D, Granowitz E V, Dinarello C A

机构信息

Dept. of Medicine, New England Medical Center, Boston, MA 02111.

出版信息

J Infect Dis. 1993 Jun;167(6):1344-50. doi: 10.1093/infdis/167.6.1344.

DOI:10.1093/infdis/167.6.1344

PMID:8388902

Abstract

The tumor necrosis factor (TNF) soluble receptor derived from the cell surface p55 TNF receptor (TNFsRp55) is a naturally occurring substance generated during infection and inflammation. TNFsRp55 inhibits biologic effects of TNF. An RIA was developed to quantitate TNFsRp55 in human blood. Recovery of TNFsRp55 from blood anticoagulated with EDTA was optimal compared with recovery from serum or heparinized plasma. TNF did not interfere with the assay. With the RIA based on radiolabeled nonglycosylated Escherichia coli-derived recombinant TNFsRp55, a mean concentration of 198 +/- 15 pg/mL was found in 14 volunteers. When glycosylated CHO cell-derived TNFsRp55 was used, the mean level was 1656 +/- 95 pg/mL. Infusion of endotoxin into volunteers induced TNFsRp55, which peaked at 517 +/- 99 pg/mL for the E. coli-based RIA and 7300 +/- 1810 pg/mL for the CHO cell-based RIA. These findings demonstrate that blood collected in EDTA is optimal for measuring circulating TNFsRp55 and that this soluble receptor is present in health but elevated during endotoxemia.

摘要

肿瘤坏死因子（TNF）可溶性受体源自细胞表面的p55 TNF受体（TNFsRp55），是感染和炎症期间产生的一种天然物质。TNFsRp55可抑制TNF的生物学效应。已开发出一种放射免疫分析法（RIA）来定量检测人血液中的TNFsRp55。与从血清或肝素化血浆中回收相比，用乙二胺四乙酸（EDTA）抗凝的血液中TNFsRp55的回收率最佳。TNF不干扰该检测方法。基于放射性标记的非糖基化大肠杆菌衍生重组TNFsRp55的RIA检测发现，14名志愿者的平均浓度为198±15 pg/mL。当使用糖基化中国仓鼠卵巢（CHO）细胞衍生的TNFsRp55时，平均水平为1656±95 pg/mL。向志愿者体内注入内毒素可诱导TNFsRp55产生，基于大肠杆菌的RIA检测其峰值为517±99 pg/mL，基于CHO细胞的RIA检测其峰值为7300±1810 pg/mL。这些发现表明，用EDTA抗凝采集的血液最适合用于检测循环中的TNFsRp55，并且这种可溶性受体在健康状态下存在，但在内毒素血症期间会升高。