Mak C, van Kooij R J, Eimers J M, te Velde E R
Department of Reproductive Medicine, University Hospital Utrecht, The Netherlands.
Andrologia. 1994 Nov-Dec;26(6):323-9. doi: 10.1111/j.1439-0272.1994.tb00811.x.
The relationship between sperm movement characteristics obtained by computerized analysis and the in vitro fertilization rates of human oocytes was studied. In 144 consecutive in vitro fertilization treatments a sample of prepared semen was analysed by a Hamilton-Thorn Motility Analyzer. In addition a visual estimation of sperm count and motility was made. Significant correlations with the fertilization rate were found for all visual parameters. Of the computerized measurements, the mean velocities of motile spermatozoa and the concentration of motile cells were significantly correlated. The average path velocity correlated best (r = 0.42, P < 0.001). There was no relationship between the percentage of motile sperm showing hyperactivated movement and the fertilization rate. A forward stepwise logistic regression analysis selected the following variables of predictive value for fertilization: average path velocity, male factor infertility as indication for in vitro fertilization, motility and concentration, as measured by the Hamilton-Thorn analyzer. A logistic regression model to predict the cases with low (< 0.2) or high fertilization rates, included the average path velocity as a significant variable and classified the samples with 90% overall accuracy.
movement characteristics of spermatozoa in culture medium, especially the average path velocity are of prognostic value in prediction of human oocyte fertilization rates.
研究了通过计算机分析获得的精子运动特征与人类卵母细胞体外受精率之间的关系。在连续144次体外受精治疗中，用汉密尔顿-桑恩运动分析仪对制备好的精液样本进行分析。此外，还对精子数量和活力进行了目测评估。发现所有目测参数与受精率均有显著相关性。在计算机测量中，活动精子的平均速度和活动细胞浓度有显著相关性。平均路径速度的相关性最佳（r = 0.42，P < 0.001）。表现出超激活运动的活动精子百分比与受精率之间没有关系。向前逐步逻辑回归分析选择了以下对受精有预测价值的变量：平均路径速度、作为体外受精指征的男性因素不育、汉密尔顿-桑恩分析仪测量的活力和浓度。一个预测低（< 0.2）或高受精率病例的逻辑回归模型，将平均路径速度作为一个显著变量，对样本进行分类，总体准确率为90%。
培养基中精子的运动特征，尤其是平均路径速度，对预测人类卵母细胞受精率具有预后价值。
